Università degli Studi della Campania "Luigi Vanvitelli," Dipartimento Multidisciplinare di Speciali, Italy.
Università della Tuscia, Dipartimento di Scienze Ecologiche e Biologiche, Viterbo, Italy.
J Biomed Opt. 2017 Nov;22(11):1-10. doi: 10.1117/1.JBO.22.11.115001.
Gingival crevicular fluid (GCF) is a site-specific exudate in the gingival sulcus. GCF composition changes in response to diseases or mechanical stimuli, such as those occurring during orthodontic treatments. Raman microspectroscopy (μ-RS) and surface-enhanced Raman spectroscopy (SERS) were adopted for a GCF analysis during different initial phases of orthodontic force application. GCF samples were pooled from informed patients using paper cones. SERS spectra were obtained from GCF extracted from these cones, whereas μ-RS spectra were directly acquired on paper cones without any manipulation. The spectral characteristics of the main functional groups and the changes in cytochrome, amide III, and amide I contributions were highlighted in the different phases of orthodontic treatment with both SERS and μ-RS analysis. μ-RS directly performed on the paper cones together with proper statistical methods can offer an effective approach for the development of a tool for monitoring the processes occurring during orthodontic treatments, which may help the clinician in the choice of type of treatment individually for each patient and accelerate and improve the orthodontic therapy.
牙龈沟液（GCF）是牙龈沟内的一种特定部位渗出液。GCF 的组成会因疾病或机械刺激而发生变化，例如在正畸治疗过程中发生的变化。拉曼微光谱（μ-RS）和表面增强拉曼光谱（SERS）被用于分析正畸力应用的不同初始阶段的 GCF。使用纸锥从知情患者中汇集 GCF 样本。从这些锥中提取的 GCF 获得了 SERS 光谱，而 μ-RS 光谱则直接在未经任何处理的纸锥上获得。SERS 和 μ-RS 分析均突出显示了正畸治疗不同阶段的主要功能基团的光谱特征以及细胞色素、酰胺 III 和酰胺 I 贡献的变化。直接在纸锥上进行 μ-RS 分析并结合适当的统计方法，可以为开发一种用于监测正畸治疗过程中发生的情况的工具提供有效方法，这可能有助于临床医生为每个患者选择个性化的治疗类型，并加速和改善正畸治疗。
