Flores-Guerrero Jose L, Muñoz-Morales Aaron, Narea-Jimenez Freddy, Perez-Fuentes Ricardo, Torres-Rasgado Enrique, Ruiz-Vivanco Guadalupe, Gonzalez-Viveros Naara, Castro-Ramos Jorge
Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
Department of Physics, Faculty of Experimental Sciences and Technology, Center of Medical and Biotechnology Research, University of Carabobo, Valencia 2005, Venezuela.
Diagnostics (Basel). 2020 Mar 3;10(3):141. doi: 10.3390/diagnostics10030141.
. Urinary albumin excretion remains the key biomarker to detect renal complications in type 2 diabetes. As diabetes epidemy increases, particularly in low-income countries, efficient and low-cost methods to measure urinary albumin are needed. In this pilot study, we evaluated the performance of Raman spectroscopy in the assessment of urinary albumin in patients with type 2 diabetes. The spectral Raman analysis of albumin was performed using artificial urine, at five concentrations of albumin and 24 h collection urine samples from ten patients with Type 2 Diabetes. The spectra were obtained after removing the background fluorescence and fitting Gaussian curves to spectral regions containing features of such metabolites. In the samples from patients with type 2 diabetes, we identified the presence of albumin in the peaks of the spectrum located at 663.07, 993.43, 1021.43, 1235.28, 1429.91 and 1633.91 cm. In artificial urine, there was an increase in the intensity of the Raman signal at 1450 cm, which corresponds to the increment of the concentrations of albumin. The highest concentration of albumin was located at 1630 cm. The capability of Raman spectroscopy for detection of small concentrations of urinary albumin suggests the feasibility of this method for the screening of type 2 diabetes renal complications.
尿白蛋白排泄仍然是检测2型糖尿病肾脏并发症的关键生物标志物。随着糖尿病流行率的增加，特别是在低收入国家，需要高效且低成本的方法来测量尿白蛋白。在这项初步研究中，我们评估了拉曼光谱法在评估2型糖尿病患者尿白蛋白方面的性能。使用人工尿液，在五种白蛋白浓度下以及从10名2型糖尿病患者收集的24小时尿液样本上进行白蛋白的拉曼光谱分析。在去除背景荧光并对包含此类代谢物特征的光谱区域拟合高斯曲线后获得光谱。在2型糖尿病患者的样本中，我们在位于663.07、993.43、1021.43、1235.28、1429.91和1633.91 cm的光谱峰中鉴定出白蛋白的存在。在人工尿液中，1450 cm处的拉曼信号强度增加，这与白蛋白浓度的增加相对应。白蛋白的最高浓度位于1630 cm处。拉曼光谱法检测低浓度尿白蛋白的能力表明该方法用于筛查2型糖尿病肾脏并发症的可行性。
