Consiglio Nazionale delle Ricerche, SPIN-CNR, 80078 Pozzuoli, Italy.
Dipartimento di Fisica "E. Pancini", Università "Federico II" di Napoli, 80126 Naples, Italy.
Sensors (Basel). 2018 Jul 12;18(7):2242. doi: 10.3390/s18072242.
Graphene provides a unique way of sensing the local pH level of substances on the micrometric scale, with important implications for the monitoring of cellular metabolic activities where proton excretion could occur. Accordingly, an innovative biosensing approach for the quantification of the pH value of biological fluids, to be used also with small amounts of fluids, was realized and tested. It is based on the use of micro-Raman spectroscopy to detect the modifications of the graphene doping level induced by the contact of the graphene with the selected fluids. The approach was preliminarily tested on aqueous solutions of known pH values. It was then used to quantify the pH values of cell culture media directly exposed to different doses of X-ray radiation and to media exposed to X-ray-irradiated cells. The Raman response of cells placed on graphene layers was also examined.
石墨烯为在微米尺度上感知物质的局部 pH 值提供了一种独特的方法，这对于监测细胞代谢活动具有重要意义，因为细胞代谢活动中可能会有质子排出。因此，实现并测试了一种用于定量生物流体 pH 值的创新生物传感方法，该方法也可用于少量流体。它基于使用微拉曼光谱检测石墨烯与所选流体接触引起的石墨烯掺杂水平的变化。该方法首先在已知 pH 值的水溶液中进行了初步测试。然后，它被用于定量直接暴露于不同剂量 X 射线辐射的细胞培养基和暴露于 X 射线照射细胞的培养基的 pH 值。还检查了放在石墨烯层上的细胞的拉曼响应。
