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在自主细小病毒小鼠微小病毒的DNA复制中存在连接步骤的证据。

Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice.

作者信息

Cotmore S F, Gunther M, Tattersall P

机构信息

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

出版信息

J Virol. 1989 Feb;63(2):1002-6. doi: 10.1128/JVI.63.2.1002-1006.1989.

DOI:10.1128/JVI.63.2.1002-1006.1989
PMID:2911112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247784/
Abstract

Newly replicated DNA of the autonomous parvovirus minute virus of mice was pulse-labeled with 32PO4 during the time of maximal viral DNA replication in highly synchronized A9 cells. The subsequent processing of viral DNA-protein complexes was monitored during a chase period with no label. Several distinct classes of duplex replicative-form and progeny single-stranded DNA molecules were characterized and found to accumulate at different times during infection. Analysis of the terminal structures associated with these various forms provided new insights into the mechanism by which viral DNA replicates and, in particular, suggested that interstrand ligation occurs during this process.

摘要

在高度同步化的A9细胞中，当小鼠自主细小病毒微小病毒的病毒DNA进行最大程度复制时，用³²Pₒ₄对新复制的DNA进行脉冲标记。在无标记的追踪期内监测病毒DNA - 蛋白质复合物的后续处理过程。对几类不同的双链复制型和子代单链DNA分子进行了表征，发现它们在感染过程中的不同时间积累。对与这些不同形式相关的末端结构的分析为病毒DNA复制机制提供了新的见解，特别是表明在此过程中发生了链间连接。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/7e32b0515d3d/jvirol00069-0547-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/c331e1ff11ab/jvirol00069-0545-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/f7ed89c26942/jvirol00069-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/799a3338b3cc/jvirol00069-0546-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/7e32b0515d3d/jvirol00069-0547-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/c331e1ff11ab/jvirol00069-0545-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/f7ed89c26942/jvirol00069-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/799a3338b3cc/jvirol00069-0546-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af83/247784/7e32b0515d3d/jvirol00069-0547-a.jpg

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本文引用的文献

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Cloning of the initiation region of a mammalian chromosomal replicon.哺乳动物染色体复制子起始区域的克隆
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Analysis of bacteriophage phi X174 gene A protein-mediated termination and reinitiation of phi X DNA synthesis. II. Structural characterization of the covalent phi X A protein-DNA complex.
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Specific initiation of replication at the right-end telomere of the closed species of minute virus of mice replicative-form DNA.小鼠微小病毒复制型DNA封闭种右端端粒处复制的特异性起始
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