Gunther M, Tattersall P
Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510.
FEBS Lett. 1988 Dec 19;242(1):22-6. doi: 10.1016/0014-5793(88)80977-3.
A new assay (label transfer from DNA to protein) enabled the identification of a terminal protein (TP) in nucleoprotein complexes extracted from cells infected with the parvovirus, minute virus of mice, MVM. In SDS-PAGE, TP migrates as a major band at 83 kDa, with a minor 65 kDa component, each of which exactly co-migrates with the cellular forms of the virally coded polypeptide NS-1. In parallel, the analysis of nucleoproteins by SDS-agarose gel electrophoresis allowed us to observe that the major species of viral DNA molecules (mRF, dRF and ssDNA) are all present in the form of DNA-protein complexes. Three forms of mRF DNA were identified, two of which are protein-associated and one which appears to be protein-free.
一种新的检测方法（从DNA到蛋白质的标记转移）能够鉴定从小鼠细小病毒MVM感染的细胞中提取的核蛋白复合物中的末端蛋白（TP）。在SDS-PAGE中，TP以83 kDa的主要条带迁移，还有一个65 kDa的次要成分，它们各自与病毒编码的多肽NS-1的细胞形式精确共迁移。同时，通过SDS-琼脂糖凝胶电泳对核蛋白的分析使我们观察到病毒DNA分子的主要种类（mRF、dRF和ssDNA）均以DNA-蛋白质复合物的形式存在。鉴定出了三种形式的mRF DNA，其中两种与蛋白质相关，一种似乎不含蛋白质。
