埃及孕妇和绵羊中弓形虫的分子和血清学流行情况
Molecular and serological prevalence of Toxoplasma gondii in pregnant women and sheep in Egypt.
作者信息
Ibrahim Hany M, Mohamed Azza H, El-Sharaawy Ahmed A, El-Shqanqery Hend E
机构信息
Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.
Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.
出版信息
Asian Pac J Trop Med. 2017 Oct;10(10):996-1001. doi: 10.1016/j.apjtm.2017.09.012. Epub 2017 Sep 19.
OBJECTIVE
To investigate molecular and serological prevalence of Toxoplasma gondii (T. gondii) in pregnant women and sheep in Egypt.
METHODS
Blood samples collected from healthy 364 pregnant women and 170 sheep were investigated for T. gondii antibodies and parasitemia using highly specific and sensitive surface antigen (TgSAG2) based enzyme linked immunosorbent assay (ELISA) and real time-polymerase chain reaction (RT-PCR).
RESULTS
Overall prevalence of T. gondii was 51.76%, 17.65% in sheep, 33.79%, 11.81% in pregnant women, using ELISA and RT-PCR respectively. Significant differences in T. gondii prevalence were observed on the basis of contact with cats or soil in pregnant women using either RT-PCR or ELISA. In pregnant women, a significant increase was detected in aged and those eating under-cooked mutton using simultaneous ELISA/RT-PCR.
CONCLUSIONS
Consumption of under-cooked infected mutton is an important source of human infection and the combination of the two assays provide accurate and precise data during infection.
目的
调查埃及孕妇和绵羊中弓形虫的分子和血清学流行情况。
方法
采用基于高特异性和敏感性表面抗原（TgSAG2）的酶联免疫吸附测定（ELISA）和实时聚合酶链反应（RT-PCR），对从364名健康孕妇和170只绵羊采集的血样进行弓形虫抗体和寄生虫血症检测。
结果
使用ELISA和RT-PCR检测，弓形虫的总体流行率分别为51.76%，绵羊为17.65%，孕妇为33.79%，使用RT-PCR时孕妇为11.81%。在孕妇中，无论使用RT-PCR还是ELISA，根据与猫或土壤的接触情况观察到弓形虫流行率存在显著差异。在孕妇中，同时使用ELISA/RT-PCR检测发现，年龄较大者和食用未煮熟羊肉者的感染率显著增加。
结论
食用未煮熟的受感染羊肉是人类感染的重要来源，两种检测方法的联合使用可在感染期间提供准确精确的数据。
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