Kim Ji-Young, Park So-Hyun, Lee Dong-Heon, Song Gwanpil, Kim Young-Ju
Research institute for basic science, Jeju National University, Jeju 63241, Republic of Korea.
Department of Aquatic Life Medicine, College of Ocean Science, Jeju 63241, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Jan;68(1):9-13. doi: 10.1099/ijsem.0.002294. Epub 2017 Nov 7.
A sulfur-oxidizing bacterium, designated strain KBB12, was isolated from swinery waste collected in Jeju, Republic of Korea. The cells were Gram-stain-negative, flagellated and rod-shaped. Growth occurred at 15-45 °C (optimum, 30-37 °C), at pH 6-9 (optimum, pH 7.0) and in the presence of 0-1 % (w/v) NaCl. The major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c, C16 : 0 and C18 : 1ω7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid and an unidentified lipid. The major isoprenoid quinone was ubiquinone-8 (Q-8) and the DNA G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate belongs to the genus Melaminivora and was most closely related to Melaminivora alkalimesophila CY1 (97.2 % similarity). The DNA-DNA relatedness values between strain KBB12 and M. alkalimesophila DSM26005 was 43.4±2.7 %. On the basis of phylogenetic and phenotypic evidence, it is proposed that strain KBB12 represents a novel species of the genus Melaminivora, for which the name Melaminivora jejuensis sp. nov. is proposed. The type strain is KBB12 (=KCTC 32230=JCM 18740).
从韩国济州岛收集的猪场废弃物中分离出一株硫氧化细菌，命名为菌株KBB12。细胞革兰氏染色阴性，具鞭毛，呈杆状。在15 - 45 °C（最适温度为30 - 37 °C）、pH 6 - 9（最适pH为7.0）以及0 - 1 %（w/v）NaCl存在的条件下生长。主要细胞脂肪酸为总和特征3（异-C15 : 0 2-OH和/或C16 : 1 ω7c、C16 : 0和C18 : 1ω7c）。极性脂质为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂和一种未鉴定的脂质。主要类异戊二烯醌为泛醌-8（Q-8），基因组DNA的G+C含量为69.6 mol%。基于16S rRNA基因序列的系统发育分析表明，该新分离菌株属于Melaminivora属，与嗜碱Melaminivora alkalimesophila CY1关系最为密切（相似性为97.2 %）。菌株KBB12与嗜碱Melaminivora alkalimesophila DSM26005之间的DNA-DNA相关性值为43.4±2.7 %。基于系统发育和表型证据，提议菌株KBB12代表Melaminivora属的一个新物种，命名为济州Melaminivora jejuensis sp. nov.。模式菌株为KBB12（=KCTC 32230=JCM 18740）。
