Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005701.
A bacterial strain designated SC2-9 was isolated from the dust collector of a pigpen located in Wanju-gun, Jeollabuk-do, Republic of Korea. Cells were strictly aerobic, Gram-stain-negative, flagellated and rod-shaped. The strain was catalase- and oxidase-positive, and grew optimally 28-30 °C, pH 8.0 and 0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed 99.1 and 98.3 % similarities to KBB12 and CY1, and revealing less than 97 % similarity to other validly named species. The genomic DNA G+C content of strain SC2-9 was 68.2 %. The orthologous average nucleotide identity and dDDH values of strain SC2-9 with the closest species KCTC 32230 were 85.6 and 29.3 %, respectively. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids and one unidentified lipid. The major fatty acids (>10 %) were summed feature 3 (C 6 and/or C 7), C and summed feature 8 (C 6 and/ or C 7). The predominant isoprenoid quinone was ubiquinone-8. Based on phenotypic, chemotaxonomic and phylogenetic data, strain SC2-9 should be assigned as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SC2-9 (=KACC 19310=NBRC 113103).
一株编号为 SC2-9 的细菌菌株从位于韩国全罗北道完州郡的猪圈集尘器中分离得到。细胞为严格需氧、革兰氏阴性、鞭毛和杆状。该菌株过氧化氢酶和氧化酶阳性，最佳生长温度为 28-30°C，pH 值为 8.0，NaCl（w/v）含量为 0%。基于 16S rRNA 基因序列的系统发育分析显示，该菌株与 KBB12 和 CY1 的相似度分别为 99.1%和 98.3%，与其他有效命名的种的相似度均小于 97%。菌株 SC2-9 的基因组 DNA G+C 含量为 68.2%。与最接近的种 KCTC 32230 的直系同源平均核苷酸同一性和 dDDH 值分别为 85.6%和 29.3%。极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、三种未鉴定的胺脂和一种未鉴定的脂。主要脂肪酸（>10%）为(summed feature 3，C 6 和/或 C 7)、C 和(summed feature 8，C 6 和/或 C 7)。主要的异戊二烯醌为泛醌-8。基于表型、化学分类和系统发育数据，菌株 SC2-9 应被归为属的一个新种，提议命名为 sp. nov.。模式菌株为 SC2-9（=KACC 19310=NBRC 113103）。
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