Berneman Z N, Chen Z Z, Ramael M, Van Hoof V, Peetermans M E
Laboratory of Experimental Hematology, University of Antwerp, Belgium.
Leukemia. 1989 Feb;3(2):151-4.
Human long-term bone marrow cultures (HLTBMCs) were established with bone marrow (BM) collected from five patients with myelomatous disorders (four with multiple myeloma, one with plasma cell leukemia). In all cases, up to at least 6 weeks of culture, there was a persistence of the monoclonal plasma cell population in the adherent layer of the culture. In some cultures proliferating plasma cells could be demonstrated by the Ki-67 monoclonal antibody. In all instances a paraprotein could be shown in the conditioned medium. This study demonstrates that malignant plasma cells, in analogy to their normal counterparts, have an affinity for the BM stroma and suggests that their long-term survival might be enhanced by their interaction with it.
用人骨髓瘤疾病患者（4例多发性骨髓瘤、1例浆细胞白血病）的骨髓建立人长期骨髓培养（HLTBMC）。在所有病例中，培养至少6周，培养物贴壁层中均持续存在单克隆浆细胞群。在一些培养物中，增殖的浆细胞可用Ki-67单克隆抗体显示。在所有情况下，条件培养基中均可显示副蛋白。本研究表明，恶性浆细胞与其正常对应物类似，对骨髓基质具有亲和力，并提示其与骨髓基质的相互作用可能会提高其长期存活率。
