Klimovich V B, Samoĭlovich M P
Tsitologiia. 1985 Nov;27(11):1215-27.
Current knowledge of primary culturing and establishment of continuous cell lines from human plasmacytomas are reviewed. The cells derived from chronic monoclonal tumors are capable of proliferating in culture no more than for 3 weeks. The enrichment of the media with nutrient supplements and growth factors does not improve the cell survival. Most of the described stable cell lines were derived from malignancies of the late stages of tumor progression. Cell lines producing complete molecules of immunoglobulins were established with the aid of sophisticated culturing methods. The microenvironment and growth factors in culture system favoured the survival of transformed plasma cells which retained a high level of differentiation.
本文综述了人浆细胞瘤原代培养及连续细胞系建立的当前知识。源自慢性单克隆肿瘤的细胞在培养中增殖能力不超过3周。用营养补充剂和生长因子富集培养基并不能提高细胞存活率。大多数已描述的稳定细胞系源自肿瘤进展后期的恶性肿瘤。借助复杂的培养方法建立了产生完整免疫球蛋白分子的细胞系。培养系统中的微环境和生长因子有利于保留高水平分化的转化浆细胞的存活。
