Department of Horticulture, National Ilan University, Yilan City 260, Taiwan.
Department of Life Sciences, National University of Kaohsiung, Kaohsiung 811, Taiwan.
Molecules. 2017 Nov 7;22(11):1907. doi: 10.3390/molecules22111907.
This study developed an efficient and reliable system for inducing polyploidy in Hayata, a top-grade medicinal orchid. The resulting tetraploid gave a significant enhancement on various agronomic traits, including dry weight, fresh weight, shoot length, root length, leaf width, the size of stoma, and number of chloroplasts per stoma. A reduction of the ratio of length to width was observed in stomata and leaves of the tetraploid, and consequently, an alteration of organ shape was found. The major bioactive compounds, total flavonoid and gastrodin, were determined by the aluminum chloride colorimetric method and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), respectively. The tetraploid produced significantly higher contents of total flavonoid and gastrodin in the leaf, the stem, and the whole plant when compared with the diploid. The resulting tetraploids in this study are proposed to be suitable raw materials in the pharmaceutical industry for enhancing productivity and reducing cost.
本研究开发了一种高效可靠的方法，用于诱导优质药用兰花 Hayata 产生多倍体。所得的四倍体在各种农艺性状上有显著提高，包括干重、鲜重、茎长、根长、叶宽、气孔大小和每个气孔中的叶绿体数量。四倍体气孔和叶片的长宽比减小，从而发现器官形状发生了改变。采用三氯化铝比色法和超高效液相色谱串联质谱法（UPLC-MS/MS）分别测定主要生物活性化合物总黄酮和天麻素的含量。与二倍体相比，四倍体在叶片、茎和整株植物中的总黄酮和天麻素含量显著增加。本研究中的四倍体被提议作为制药工业中提高生产力和降低成本的合适原料。
