Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.
CNT-Application Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.
Sensors (Basel). 2017 Nov 7;17(11):2569. doi: 10.3390/s17112569.
The photoluminescence properties of carbon nanotubes (CNTs), including the large Stokes shift and the absence of fluorescent photobleaching, can be used as a fluorescent label in biological measurements. In this study, the performance of CNTs as a fluorescent label for surface plasmon resonance (SPR)-assisted fluoroimmunoassay is evaluated. The fluorescence of (8, 3) CNTs with an excitation wavelength of 670 nm and an emission wavelength of 970 nm is observed using a sensor chip equipped with a prism-integrated microfluidic channel to excite the SPR. The minimum detectable concentration of a CNT dispersed in water using a visible camera is 0.25 μg/mL, which is equivalent to 2 × 10 tubes/mL. The target analyte detection using the CNT fluorescent labels is theoretically investigated by evaluating the detectable number of CNTs in a detection volume. Assuming detection of virus particles which are bound with 100 CNT labels, the minimum number of detectable virus particles is calculated to be 900. The result indicates that CNTs are effective fluorescent labels for SPR-assisted fluoroimmunoassay.
碳纳米管（CNTs）的光致发光性能，包括大斯托克斯位移和荧光漂白缺失，可作为生物测量中的荧光标记物。本研究评估了 CNTs 作为表面等离子体共振（SPR）辅助荧光免疫测定中荧光标记物的性能。使用配备有棱镜集成微流道的传感器芯片激发 SPR，观察到激发波长为 670nm、发射波长为 970nm 的（8,3）CNTs 的荧光。使用可见相机分散在水中的 CNT 的最低可检测浓度为 0.25μg/mL，相当于 2×10 管/mL。通过评估检测体积中可检测到的 CNT 数量，从理论上研究了使用 CNT 荧光标记物进行目标分析物检测。假设检测与 100 个 CNT 标记物结合的病毒颗粒，则计算出的最小可检测病毒颗粒数为 900。结果表明，CNTs 是 SPR 辅助荧光免疫测定的有效荧光标记物。
