1] Flexible Electronics Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan [2].
1] Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan [2].
Nat Commun. 2013;4:2855. doi: 10.1038/ncomms3855.
Surface plasmon excitation provides stronger enhancement of the fluorescence intensity and better sensitivity than other sensing approaches but requires optimal positioning of a prism to ensure optimum output of the incident light. Here we describe a simple, highly sensitive optical sensing system combining surface plasmon excitation and fluorescence to address this limitation. V-shaped fluidic channels are employed to mimic the functions of a prism, sensing plate, and flow channel in a single setup. Superior performance is demonstrated for different biomolecular recognition reactions on a self-assembled monolayer, and the sensitivity reaches 100 fM for biotin-streptavidin interactions. Using an antibody as a probe, we demonstrate the detection of intact influenza viruses at 0.2 HA units ml⁻¹ levels. The convenient sensing system developed here has the advantages of being prism-free and requiring less sample (1-2 μl), making this platform suitable for use in situations requiring low sample volumes.
表面等离子体激元激发比其他传感方法提供更强的荧光强度增强和更好的灵敏度，但需要最佳定位棱镜以确保最佳的入射光输出。在这里，我们描述了一种简单、高灵敏度的光学传感系统，结合表面等离子体激元和荧光来解决这个限制。V 形流体通道被用来模拟棱镜、传感板和流道的功能，在单个设置中。在自组装单层上进行不同的生物分子识别反应时，表现出优异的性能，并且对于生物素-链霉亲和素相互作用的灵敏度达到 100 fM。使用抗体作为探针，我们证明了在 0.2 HA 单位 ml⁻¹ 水平下对完整流感病毒的检测。这里开发的方便传感系统具有无棱镜和需要更少样品（1-2 μl）的优点，使得该平台适用于需要低样品体积的情况。
