Zhang Fumin, Wan Ming, Xu Yi, Li Zhenglong, Kang Pengcheng, Jiang Xingming, Wang Yimin, Wang Zhidong, Zhong Xiangyu, Li Chunlong, Cui Yunfu
Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150000, P.R. China.
Department of General Surgery, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163001, P.R. China.
Oncol Lett. 2017 Nov;14(5):6079-6084. doi: 10.3892/ol.2017.6987. Epub 2017 Sep 18.
The incidence of extrahepatic cholangiocarcinoma (ECC) is the highest of all the cholangiocarcinoma cases. However, the molecular mechanism of ECC genesis and progression remains unclear. Long non-coding RNAs (lncRNAs) have been revealed to perform critical regulatory roles in cancer biology. In order to understand lncRNA expression patterns and their potential function in ECC, a transcriptome analysis of lncRNA and mRNA expression was performed in ECC and paired adjacent non-cancerous tissues using Agilent human lncRNA + mRNA arrayV4.0 (4×180 K format). It was identified that 268 lncRNAs and 459 mRNAs were differentially expressed in ECC. Among these, 78 lncRNAs and 66 mRNAs were upregulated >2-fold compared with adjacent non-cancerous tissues, and 190 lncRNAs and 393 mRNAs were downregulated in the ECC samples. Differences in lncRNA expression between ECC and paired adjacent non-cancerous tissues were confirmed using reverse transcription-quantitative polymerase chain reactionas proof of principle. Functional analysis of co-expressed mRNAs with lncRNAs indicated that these dysregulated lncRNAsmay be involved in known ECC-associated biological processes and pathways. The present findings indicated that mRNAs and lncRNAs perform important roles in the development and progression of ECC. The present findings may lay the foundation for future efforts to understand the role of lncRNAs and develop novel biomarkers in ECC.
肝外胆管癌（ECC）的发病率在所有胆管癌病例中最高。然而，ECC发生和进展的分子机制仍不清楚。长链非编码RNA（lncRNA）已被揭示在癌症生物学中发挥关键的调节作用。为了了解lncRNA在ECC中的表达模式及其潜在功能，使用安捷伦人类lncRNA + mRNA阵列V4.0（4×180 K格式）对ECC及配对的相邻非癌组织进行了lncRNA和mRNA表达的转录组分析。结果发现，268个lncRNA和459个mRNA在ECC中差异表达。其中，与相邻非癌组织相比，78个lncRNA和66个mRNA上调超过2倍，190个lncRNA和393个mRNA在ECC样本中下调。使用逆转录定量聚合酶链反应作为原理验证，证实了ECC与配对的相邻非癌组织之间lncRNA表达的差异。对与lncRNA共表达的mRNA进行功能分析表明，这些失调的lncRNA可能参与已知的ECC相关生物学过程和途径。本研究结果表明，mRNA和lncRNA在ECC的发生和进展中发挥重要作用。本研究结果可能为未来了解lncRNA的作用以及开发ECC新型生物标志物奠定基础。
