Mignotte V, Wall L, deBoer E, Grosveld F, Romeo P H
Laboratory of Gene Structure and Expression, National Institute for Medical Research, London, UK.
Nucleic Acids Res. 1989 Jan 11;17(1):37-54. doi: 10.1093/nar/17.1.37.
We have studied the erythroid-specific promoter of the human gene coding for Porphobilinogen Deaminase (PBGD) by DNaseI footprinting, gel retardation and methylation interference assays. We show that this promoter, which is inducible during MEL cell differentiation, contains three binding sites for the erythroid-specific factor NF-E1 and one site for a second erythroid-specific factor, which we name NF-E2. NF-E1 is a factor that also binds the promoter and the enhancer (present in the 3' flanking region) of the human beta-globin gene. NF-E2 has not yet been described and although it binds to a sequence containing the Ap1 consensus, it appears to be different from Ap1.
我们通过DNA酶I足迹法、凝胶阻滞试验和甲基化干扰试验研究了编码胆色素原脱氨酶（PBGD）的人类基因的红系特异性启动子。我们发现，这个在MEL细胞分化过程中可诱导的启动子，包含三个红系特异性因子NF-E1的结合位点和一个第二个红系特异性因子的结合位点，我们将其命名为NF-E2。NF-E1也是一种能与人β-珠蛋白基因的启动子和增强子（位于3'侧翼区域）结合的因子。NF-E2尚未见报道，尽管它能与包含Ap1共有序列的序列结合，但它似乎与Ap1不同。
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