一种通过在聚合酶链反应（PCR）中使用单一特异性寡核苷酸和寡聚（dT）进行直接克隆和测序cDNA的简单方法。 - Suppr

Suppr

超能文献

A simple method for direct cloning and sequencing cDNA by the use of a single specific oligonucleotide and oligo(dT) in a polymerase chain reaction (PCR).

作者信息

Berchtold M W

机构信息

Institute of Pharmacology and Biochemistry, University Zürich-Irchel, Switzerland.

出版信息

Nucleic Acids Res. 1989 Jan 11;17(1):453. doi: 10.1093/nar/17.1.453.

DOI:10.1093/nar/17.1.453

PMID:2911482

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331573/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67f6/331573/ce0d060c4d24/nar00210-0456-a.jpg

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本文引用的文献

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。

Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.

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