Department of General Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
Mol Med Rep. 2018 Jan;17(1):51-58. doi: 10.3892/mmr.2017.7846. Epub 2017 Oct 20.
Evidence has suggested that salidroside inhibits the proliferation and invasion of renal clear cell, lung, breast, and colon cancer. However, effect of salidroside on colorectal cancer (CRC) cells against oxaliplatin (L‑OHP) resistance remains unclear. In the present study, the CRC HT‑29 cell line and L‑OHP resistance HT‑29/L‑OHP cell line were used to evaluate the effect, and mechanism of salidroside on L‑OHP resistance. The results demonstrated that the activity of HT‑29 cells was lower compared with that of HT‑29/L‑OHP cells following L‑OHP intervention, and was accompanied with varied expression levels of drug resistant proteins. The combination of salidroside and L‑OHP weakened cell activity significantly compared single utilization. Compared with the control group, salidroside intervention resulted in a higher percentage of HT‑29/L‑OHP cells in the G0/G1 stage, and reduced percentage in the G2/M stage, but no significant variation in the S stage. The HT‑29/L‑OHP cells exhibited increased apoptosis rates and caspase‑3 activity, but decreased metastatic, and invasive abilities following salidroside intervention. Quantitative polymerase chain reaction and western blot analysis detected variations in the expression levels of associated genes in HT‑29/L‑OHP cells following salidroside intervention. In all, the results of the present study revealed that salidroside is able to decrease the activity and invasive capacity of HT‑29/L‑OHP cells, and treatment with salidroside is associated with increased apoptosis of cancer cells through the regulation of certain genes.
有证据表明，红景天苷可抑制肾透明细胞癌、肺癌、乳腺癌和结肠癌的增殖和侵袭。然而，红景天苷对结直肠癌细胞（CRC）对抗奥沙利铂（L-OHP）耐药性的影响尚不清楚。本研究采用 CRC HT-29 细胞系和 L-OHP 耐药 HT-29/L-OHP 细胞系，评估红景天苷对 L-OHP 耐药性的作用及其机制。结果表明，L-OHP 干预后 HT-29 细胞的活性低于 HT-29/L-OHP 细胞，且耐药蛋白的表达水平也存在差异。与单独使用相比，红景天苷和 L-OHP 的联合使用显著减弱了细胞活性。与对照组相比，红景天苷干预后 HT-29/L-OHP 细胞 G0/G1 期比例升高，G2/M 期比例降低，但 S 期无明显变化。红景天苷干预后 HT-29/L-OHP 细胞凋亡率和 caspase-3 活性增加，但迁移和侵袭能力降低。定量聚合酶链反应和 Western blot 分析检测到 HT-29/L-OHP 细胞中相关基因表达水平的变化。综上所述，本研究结果表明，红景天苷能够降低 HT-29/L-OHP 细胞的活性和侵袭能力，红景天苷治疗与通过调节某些基因增加癌细胞凋亡有关。
