• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

微小RNA-20a-5p通过靶向SDC2基因抑制骨肉瘤的多药耐药性。

MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene.

作者信息

Zhao Fangfang, Pu Youguang, Cui Mingda, Wang Haiyan, Cai Shanbao

机构信息

Cancer Epigenetics Program, Anhui Cancer Hospital, West Branch of Anhui Provincial Hospital, Anhui Medical University, Hefei, 230031 Anhui China.

Xinxiang Medical University, Xinxiang, 453003 Henan China.

出版信息

Cancer Cell Int. 2017 Nov 2;17:100. doi: 10.1186/s12935-017-0470-2. eCollection 2017.

DOI:10.1186/s12935-017-0470-2
PMID:29118673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5668954/
Abstract

BACKGROUND

As one of the hallmarks of cancer, chemoresistance hinders curative cancer chemotherapy in osteosarcoma (OS). MicroRNAs (miRNAs) act as key regulators of gene expression in diverse biological processes including the multi-chemoresistance of cancers.

METHODS

Based on the CCK8 experiments, we performed an RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells (a multi-chemosensitive OS cell line G-292 and a multi-chemoresistant OS cell line SJSA-1) to detect the levels of miR-20a-5p. We predicted syndecan 2 (SDC2) as one of the target genes of miR-20a-5p via several websites, which was further validated by detecting their expression of both mRNA and protein level in both the miR-20a-5p-mimic transfected G-292 and miR-20a-5p-antagomiR transfected SJSA-1 cells. The involvement of SDC2 with OS chemoresistance was checked by siRNA-mediated repression or overexpression of SDC2 gene. Cell viability was assessed by CCK8 assay.

RESULTS

We found that the miR-20a-5p level was higher in G-292 cells than in SJSA-1 cells. Forced expression of miR-20a-5p counteracted OS chemoresistance in both cell culture and tumor xenografts in nude mice. As one of miR-20a-5p's targets, SDC2 was found to mediate the miR-20a-5p-induced repression of OS chemoresistance.

CONCLUSIONS

Our results suggest that miR-20a-5p and SDC2 contribute to OS chemoresistance. The key players in the miR-20a-5p/SDC2 axis may be a potential diagnostic biomarker and therapeutic target for OS patients.

摘要

背景

化疗耐药作为癌症的标志之一，阻碍了骨肉瘤（OS）的根治性化疗。微小RNA（miRNA）在包括癌症多药耐药在内的多种生物学过程中作为基因表达的关键调节因子。

方法

基于CCK8实验，我们对骨肉瘤（OS）细胞（多化学敏感的OS细胞系G - 292和多化学耐药的OS细胞系SJSA - 1）进行了基于RNA测序的miR组分析，以检测miR - 20a - 5p的水平。我们通过多个网站预测syndecan 2（SDC2）是miR - 20a - 5p的靶基因之一，并通过检测miR - 20a - 5p模拟物转染的G - 292细胞和miR - 20a - 5p拮抗剂转染的SJSA - 1细胞中mRNA和蛋白质水平的表达进一步验证。通过siRNA介导的SDC2基因抑制或过表达来检查SDC2与OS化疗耐药的关系。通过CCK8测定评估细胞活力。

结果

我们发现G - 292细胞中miR - 20a - 5p水平高于SJSA - 1细胞。在细胞培养和裸鼠肿瘤异种移植中，miR - 20a - 5p的强制表达均抵消了OS化疗耐药。作为miR - 20a - 5p的靶标之一，发现SDC2介导miR - 20a - 5p诱导的OS化疗耐药抑制。

结论

我们的结果表明miR - 20a - 5p和SDC2促成了OS化疗耐药。miR - 20a - 5p/SDC2轴中的关键分子可能是OS患者潜在的诊断生物标志物和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/08afc41b7ac4/12935_2017_470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/f4ecdee3fc71/12935_2017_470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/864e0099bbf7/12935_2017_470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/d24a4f46d947/12935_2017_470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/fff61e3cbb8b/12935_2017_470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/6d42858b8dc8/12935_2017_470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/08afc41b7ac4/12935_2017_470_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/f4ecdee3fc71/12935_2017_470_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/864e0099bbf7/12935_2017_470_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/d24a4f46d947/12935_2017_470_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/fff61e3cbb8b/12935_2017_470_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/6d42858b8dc8/12935_2017_470_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b3/5668954/08afc41b7ac4/12935_2017_470_Fig6_HTML.jpg

相似文献

1
MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene.微小RNA-20a-5p通过靶向SDC2基因抑制骨肉瘤的多药耐药性。
Cancer Cell Int. 2017 Nov 2;17:100. doi: 10.1186/s12935-017-0470-2. eCollection 2017.
2
MiR-20a-5p represses multi-drug resistance in osteosarcoma by targeting the KIF26B gene.微小RNA-20a-5p通过靶向KIF26B基因抑制骨肉瘤的多药耐药性。
Cancer Cell Int. 2016 Aug 5;16:64. doi: 10.1186/s12935-016-0340-3. eCollection 2016.
3
The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene.miR-34a-5p通过抑制AGTR1基因促进骨肉瘤的多药耐药性。
BMC Cancer. 2017 Jan 10;17(1):45. doi: 10.1186/s12885-016-3002-x.
4
MiR-34a-5p promotes multi-chemoresistance of osteosarcoma through down-regulation of the DLL1 gene.miR-34a-5p 通过下调 DLL1 基因促进骨肉瘤的多药耐药性。
Sci Rep. 2017 Mar 10;7:44218. doi: 10.1038/srep44218.
5
MiR-20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells.微小RNA-20a-5p通过靶向鼻咽癌细胞中的Rab27B来促进放射抗性。
Cancer Cell Int. 2017 Mar 1;17:32. doi: 10.1186/s12935-017-0389-7. eCollection 2017.
6
MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2.微小RNA-193a通过抑制胰岛素受体底物2调节人骨肉瘤细胞的化疗耐药性。
J Bone Oncol. 2019 May 11;17:100241. doi: 10.1016/j.jbo.2019.100241. eCollection 2019 Aug.
7
Syndecan-2, negatively regulated by miR-20b-5p, contributes to 5-fluorouracil resistance of colorectal cancer cells via the JNK/ERK signaling pathway.硫酸乙酰肝素蛋白聚糖 2 通过调控 miR-20b-5p 的表达促进结直肠癌细胞对 5-氟尿嘧啶的耐药性及其作用机制的研究
Acta Biochim Biophys Sin (Shanghai). 2021 Nov 10;53(11):1547-1557. doi: 10.1093/abbs/gmab124.
8
MiR-20a-5p promotes radio-resistance by targeting NPAS2 in nasopharyngeal cancer cells.微小RNA-20a-5p通过靶向鼻咽癌细胞中的NPAS2来促进放射抗性。
Oncotarget. 2017 Nov 11;8(62):105873-105881. doi: 10.18632/oncotarget.22411. eCollection 2017 Dec 1.
9
lncRNA LSINCT5 Regulates miR-20a-5p/XIAP to Inhibit the Growth and Metastasis of Osteosarcoma Cells.长链非编码RNA LSINCT5通过调控miR-20a-5p/XIAP抑制骨肉瘤细胞的生长和转移
Onco Targets Ther. 2020 Aug 18;13:8209-8221. doi: 10.2147/OTT.S251843. eCollection 2020.
10
MiR-34a-5p promotes the multi-drug resistance of osteosarcoma by targeting the CD117 gene.微小RNA-34a-5p通过靶向CD117基因促进骨肉瘤的多药耐药性。
Oncotarget. 2016 May 10;7(19):28420-34. doi: 10.18632/oncotarget.8546.

引用本文的文献

1
Comparison and Analysis of the Drug-Resistance Mechanism of Osimertinib- and Almonertinib-Resistant Cell Lines.奥希替尼和阿美替尼耐药细胞系耐药机制的比较与分析
Anal Cell Pathol (Amst). 2025 Mar 10;2025:5578693. doi: 10.1155/ancp/5578693. eCollection 2025.
2
miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL.miR-197-3p通过抑制SPOPL促进骨肉瘤干性和化疗耐药性。
J Clin Med. 2023 Feb 1;12(3):1177. doi: 10.3390/jcm12031177.
3
Advances in the Biological Functions and Mechanisms of miRNAs in the Development of Osteosarcoma.

本文引用的文献

1
MiR-20a-5p represses multi-drug resistance in osteosarcoma by targeting the KIF26B gene.微小RNA-20a-5p通过靶向KIF26B基因抑制骨肉瘤的多药耐药性。
Cancer Cell Int. 2016 Aug 5;16:64. doi: 10.1186/s12935-016-0340-3. eCollection 2016.
2
MiR-34a-5p promotes the multi-drug resistance of osteosarcoma by targeting the CD117 gene.微小RNA-34a-5p通过靶向CD117基因促进骨肉瘤的多药耐药性。
Oncotarget. 2016 May 10;7(19):28420-34. doi: 10.18632/oncotarget.8546.
3
The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation.
miRNAs 在骨肉瘤发生发展中的生物学功能及作用机制的研究进展
Technol Cancer Res Treat. 2022 Jan-Dec;21:15330338221117386. doi: 10.1177/15330338221117386.
4
Regulatory mechanism of miR-20a-5p expression in Cancer.癌症中miR-20a-5p表达的调控机制
Cell Death Discov. 2022 May 16;8(1):262. doi: 10.1038/s41420-022-01005-5.
5
microRNA-20b-5p overexpression combing Pembrolizumab potentiates cancer cells to radiation therapy via repressing programmed death-ligand 1.microRNA-20b-5p 过表达联合 Pembrolizumab 通过抑制程序性死亡配体 1 增强癌细胞对放射治疗的敏感性。
Bioengineered. 2022 Jan;13(1):917-929. doi: 10.1080/21655979.2021.2014617.
6
Circular RNA circPRKCI contributes to malignant progression of T-cell acute lymphoblastic leukemia by modulating miR-20a-5p/SOX4 axis.环状 RNA circPRKCI 通过调控 miR-20a-5p/SOX4 轴促进 T 细胞急性淋巴细胞白血病的恶性进展。
Aging (Albany NY). 2021 Oct 25;13(20):23757-23768. doi: 10.18632/aging.203647.
7
MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.miR-20a-5p 通过靶向 PPP6C 在急性髓系白血病中发挥强大的肿瘤抑制作用。
PLoS One. 2021 Sep 29;16(9):e0256995. doi: 10.1371/journal.pone.0256995. eCollection 2021.
8
Research Progress of MicroRNA in Chemotherapy Resistance of Osteosarcoma.微小 RNA 在骨肉瘤化疗耐药中的研究进展。
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211034262. doi: 10.1177/15330338211034262.
9
Curcumin attenuates Adriamycin-resistance of acute myeloid leukemia by inhibiting the lncRNA HOTAIR/miR-20a-5p/WT1 axis.姜黄素通过抑制长链非编码 RNA HOTAIR/miR-20a-5p/WT1 轴减轻阿霉素耐药的急性髓系白血病。
Lab Invest. 2021 Oct;101(10):1308-1317. doi: 10.1038/s41374-021-00640-3. Epub 2021 Jul 19.
10
Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma.靶向肝细胞癌膜跨糖蛋白 SDC4 的治疗潜力。
Cell Death Dis. 2021 May 14;12(5):492. doi: 10.1038/s41419-021-03780-y.
微小RNA-20-REST-Wnt信号轴调控神经祖细胞分化。
Sci Rep. 2016 Mar 21;6:23300. doi: 10.1038/srep23300.
4
MiR-193a-3p and miR-193a-5p suppress the metastasis of human osteosarcoma cells by down-regulating Rab27B and SRR, respectively.MiR-193a-3p和miR-193a-5p分别通过下调Rab27B和SRR来抑制人骨肉瘤细胞的转移。
Clin Exp Metastasis. 2016 Apr;33(4):359-72. doi: 10.1007/s10585-016-9783-0. Epub 2016 Feb 25.
5
MiR-20a Induces Cell Radioresistance by Activating the PTEN/PI3K/Akt Signaling Pathway in Hepatocellular Carcinoma.miR-20a 通过激活肝癌细胞中的 PTEN/PI3K/Akt 信号通路诱导细胞放射抵抗。
Int J Radiat Oncol Biol Phys. 2015 Aug 1;92(5):1132-1140. doi: 10.1016/j.ijrobp.2015.04.007. Epub 2015 Apr 13.
6
Role of syndecan-2 in osteoblast biology and pathology.Syndecan-2在成骨细胞生物学和病理学中的作用。
Bonekey Rep. 2015 Apr 1;4:666. doi: 10.1038/bonekey.2015.33. eCollection 2015.
7
Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells.人乳头瘤病毒（HPV）阳性肿瘤细胞中细胞内和外泌体微小RNA对病毒E6/E7癌基因表达的依赖性
PLoS Pathog. 2015 Mar 11;11(3):e1004712. doi: 10.1371/journal.ppat.1004712. eCollection 2015 Mar.
8
The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression.DNA甲基化调控的miR-193a-3p通过抑制SRSF2/PLAU/HIC2的表达决定膀胱癌的多药耐药性。
Cell Death Dis. 2014 Sep 4;5(9):e1402. doi: 10.1038/cddis.2014.367.
9
Hypoxic signature of microRNAs in glioblastoma: insights from small RNA deep sequencing.胶质母细胞瘤中微小RNA的缺氧特征：来自小RNA深度测序的见解
BMC Genomics. 2014 Aug 17;15(1):686. doi: 10.1186/1471-2164-15-686.
10
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.肿瘤来源的外泌体通过调控人鼻咽癌中富含的外泌体微小RNA促进肿瘤进展和T细胞功能障碍。
Oncotarget. 2014 Jul 30;5(14):5439-52. doi: 10.18632/oncotarget.2118.