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微小RNA-20a-5p通过靶向鼻咽癌细胞中的NPAS2来促进放射抗性。

MiR-20a-5p promotes radio-resistance by targeting NPAS2 in nasopharyngeal cancer cells.

作者信息

Zhao Fangfang, Pu Youguang, Qian Liting, Zang Chunbao, Tao Zhenchao, Gao Jin

机构信息

The Institute of Cancer Research, Anhui Cancer Hospital, West Branch of Anhui Provincial Hospital, Anhui Medical University, Hefei 230031, Anhui, China.

Department of Radiation Oncology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230031, Anhui, China.

出版信息

Oncotarget. 2017 Nov 11;8(62):105873-105881. doi: 10.18632/oncotarget.22411. eCollection 2017 Dec 1.

DOI:10.18632/oncotarget.22411
PMID:29285299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5739686/
Abstract

MicroRNAs (miRNAs) are key players of gene expression involved in diverse biological processes including the cancer radio-resistance, which hinders the effective cancer therapy. Here we found that the miR-20a-5p level is significantly up-regulated in radio-resistant nasopharyngeal cancer (NPC) cells via an RNA-seq and miR-omic analysis. Moreover, we identified that the neuronal PAS domain protein 2 (NPAS2) gene is one of the targets of miR-20a-5p. The involvement of miR-20a-5p and NPAS2 with NPC radio-resistance was further validated by either down- or up-regulation of their levels in NPC cell lines. Taken together, these results not only reveal novel insights into the NPC radio-resistance, but also provide hints for an effective therapeutic strategy to fight against NPC radio-resistance.

摘要

微小RNA（miRNA）是参与多种生物学过程的基因表达关键调控因子，包括癌症放射抗性，而癌症放射抗性会阻碍有效的癌症治疗。通过RNA测序和miR组分析，我们发现抗辐射鼻咽癌（NPC）细胞中miR-20a-5p水平显著上调。此外，我们确定神经元PAS结构域蛋白2（NPAS2）基因是miR-20a-5p的靶标之一。通过下调或上调NPC细胞系中miR-20a-5p和NPAS2的水平，进一步验证了它们与NPC放射抗性的关系。综上所述，这些结果不仅揭示了NPC放射抗性的新见解，也为对抗NPC放射抗性的有效治疗策略提供了线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/24869ecc03e2/oncotarget-08-105873-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/f73e67d3aadc/oncotarget-08-105873-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/8b42d6b7c0ba/oncotarget-08-105873-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/eae0d4d0a4fd/oncotarget-08-105873-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/cb24b0e1a5b3/oncotarget-08-105873-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/24869ecc03e2/oncotarget-08-105873-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/f73e67d3aadc/oncotarget-08-105873-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/8b42d6b7c0ba/oncotarget-08-105873-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/eae0d4d0a4fd/oncotarget-08-105873-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/cb24b0e1a5b3/oncotarget-08-105873-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/884f/5739686/24869ecc03e2/oncotarget-08-105873-g005.jpg

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