Elebring Erik, Kuna Vijay K, Kvarnström Niclas, Sumitran-Holgersson Suchitra
Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
The Transplant Institute, Sahlgrenska University Hospital, Gothenburg, Sweden.
J Tissue Eng. 2017 Oct 30;8:2041731417738145. doi: 10.1177/2041731417738145. eCollection 2017 Jan-Dec.
Despite progress in the field of decellularization and recellularization, the outcome for pancreas has not been adequate. This might be due to the challenging dual nature of pancreas with both endocrine and exocrine tissues. We aimed to develop a novel and efficient cold-perfusion method for decellularization of porcine pancreas and recellularize acellular scaffolds with human fetal pancreatic stem cells. Decellularization of whole porcine pancreas at 4°C with sodium deoxycholate, Triton X-100 and DNase efficiently removed cellular material, while preserving the extracellular matrix structure. Furthermore, recellularization of acellular pieces with human fetal pancreatic stem cells for 14 days showed attached and proliferating cells. Both endocrine (C-peptide and PDX1) and exocrine (glucagon and α-amylase) markers were expressed in recellularized tissues. Thus, cold-perfusion can successfully decellularize porcine pancreas, which when recellularized with human fetal pancreatic stem cells shows relevant endocrine and exocrine phenotypes. Decellularized pancreas is a promising biomaterial and might translate to clinical relevance for treatment of diabetes.
尽管在去细胞化和再细胞化领域取得了进展，但胰腺的处理结果仍不尽人意。这可能是由于胰腺具有内分泌和外分泌组织这种具有挑战性的双重性质所致。我们旨在开发一种新颖且高效的冷灌注方法，用于猪胰腺的去细胞化，并用人胎儿胰腺干细胞对脱细胞支架进行再细胞化。用脱氧胆酸钠、 Triton X - 100 和脱氧核糖核酸酶在4°C对整个猪胰腺进行去细胞化，能有效去除细胞物质，同时保留细胞外基质结构。此外，用人胎儿胰腺干细胞对脱细胞碎片进行14天的再细胞化显示细胞附着并增殖。内分泌（C肽和PDX1）和外分泌（胰高血糖素和α淀粉酶）标志物在再细胞化组织中均有表达。因此，冷灌注能够成功地使猪胰腺去细胞化，当用人胎儿胰腺干细胞进行再细胞化时，显示出相关的内分泌和外分泌表型。脱细胞胰腺是一种有前景的生物材料，可能对糖尿病治疗具有临床意义。
