Cui Ning, Wang Xiuzhen, Wang Qi, Li Hongmei, Wang Fangkun, Zhao Xiaomin
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Front Vet Sci. 2017 Oct 25;4:177. doi: 10.3389/fvets.2017.00177. eCollection 2017.
Understanding gut microflora alterations associated with gut parasites and other pathogens that drive these alterations may help to promote the understanding of intestinal flora's role in multiple-infected individuals. This study examined the effects of dual infection with and subgroup J avian leukosis virus (ALV-J) on the chick cecal microbiome. Specific-pathogen-free (SPF) chicks were infected with either ALV-J strain NX0101 at 1 day of age or at 14 days of age, another group was infected with both pathogens. Cecal contents from chicks were extracted at the 21 days of age and examined using 16S rRNA genes illumina sequencing. A genus-level opportunistic pathogen enrichment and a decrease in possible resident probiotics were observed in response to all infection groups. Of note, mainly induced a sharp decrease in the richness and diversity of cecal microflora from infected chicks because of the serious -induced damage to intestinal tissues. ALV-J infection led to structural changes and increased the richness and diversity of the cecal microflora. As for and ALV-J dual infected chicks, a marked enrichment of opportunistic pathogens in addition to some other bacteria that may play a role involving cecal microbiota carbohydrate transport and metabolic functions were also found compared to single pathogen-infected chicks. Overall, this study provides valuable insights into the SPF chick cecal microbial community, the modulations of this community in response to different pathogenic infections of single or dual infections, and the interactions between different pathogens and hosts from the perspective of intestinal microflora.
了解与肠道寄生虫及驱动这些变化的其他病原体相关的肠道微生物群变化，可能有助于增进对肠道菌群在多重感染个体中作用的理解。本研究检测了与J亚群禽白血病病毒（ALV-J）双重感染对雏鸡盲肠微生物组的影响。无特定病原体（SPF）雏鸡在1日龄时感染ALV-J毒株NX0101，或在14日龄时感染，另一组则感染两种病原体。在21日龄时提取雏鸡的盲肠内容物，并使用16S rRNA基因Illumina测序进行检测。在所有感染组中均观察到属水平的机会致病菌富集以及可能的常驻益生菌减少。值得注意的是，由于严重的感染导致肠道组织损伤，主要导致感染雏鸡盲肠微生物群的丰富度和多样性急剧下降。ALV-J感染导致结构变化，并增加了盲肠微生物群的丰富度和多样性。至于和ALV-J双重感染的雏鸡，与单一病原体感染的雏鸡相比，除了一些可能参与盲肠微生物群碳水化合物转运和代谢功能的其他细菌外，还发现机会致病菌显著富集。总体而言，本研究从肠道微生物群的角度为SPF雏鸡盲肠微生物群落、该群落对单一或双重感染的不同致病性感染的调节以及不同病原体与宿主之间的相互作用提供了有价值的见解。
