Pérez-Cataluña Alba, Tapiol Josepa, Benavent Clara, Sarvisé Carolina, Gómez Frederic, Martínez Bruno, Terron-Puig Margarida, Recio Gemma, Vilanova Angels, Pujol Isabel, Ballester Frederic, Rezusta Antonio, Figueras María Jose
Unitat de Microbiología, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
Hospital Universitari Joan XXIII, Tarragona, Spain.
J Med Microbiol. 2017 Dec;66(12):1736-1743. doi: 10.1099/jmm.0.000638. Epub 2017 Nov 9.
The genus Arcobacter includes bacteria that are considered emergent pathogens because they can produce infections in humans and animals. The most common symptoms are bloody and non-bloody persistent diarrhea but cases with abdominal cramps without diarrhea or asymptomatic cases have also been described as well as cases with bacteremia. The objective was to characterize Arcobacter clinical strains isolated from the faeces of patients from three Spanish hospitals.
We have characterized 28 clinical strains (27 of A. butzleri and one of A. cryaerophilus) isolated from faeces, analysing their epidemiological relationship using the multilocus sequence typing (MLST) approach and screening them for their antibiotic susceptibility and for the presence of virulence genes.Results/Key findings. Typing results showed that only one of the 28 identified sequence types (i.e. ST 2) was already present in the MLST database. The other 27 STs constituted new records because they included new alleles for five of the seven genes or new combinations of known alleles of the seven genes. All strains were positive for the ciaB virulence gene and sensitive to tetracycline. However, 7.4 % of the A. butzleri and A. cryaerophilus strains showed resistance to ciprofloxacin.
The fact that epidemiological unrelated strains show the same ST indicates that other techniques with higher resolution should be developed to effectively recognize the infection source. Resistance to ciprofloxacin, one of the antibiotics recommended for the treatment of Arcobacter intestinal infections, demonstrated in 10.7 % of the strains, indicates the importance of selecting the most appropriate effective treatment.
弓形杆菌属细菌被认为是新出现的病原体，因为它们可在人类和动物中引发感染。最常见的症状是血性和非血性持续性腹泻，但也有描述称出现过无腹泻的腹部绞痛病例或无症状病例以及菌血症病例。目的是对从西班牙三家医院患者粪便中分离出的弓形杆菌临床菌株进行特征分析。
我们对从粪便中分离出的28株临床菌株（27株布氏弓形杆菌和1株嗜低温弓形杆菌）进行了特征分析，采用多位点序列分型（MLST）方法分析它们的流行病学关系，并对其进行抗生素敏感性和毒力基因检测。结果/主要发现。分型结果显示，在28种已鉴定的序列类型中，只有一种（即ST 2）已存在于MLST数据库中。其他27种ST构成了新记录，因为它们包含七个基因中五个基因的新等位基因或七个基因已知等位基因的新组合。所有菌株的ciaB毒力基因均呈阳性，且对四环素敏感。然而，7.4%的布氏弓形杆菌和嗜低温弓形杆菌菌株对环丙沙星耐药。
流行病学上不相关的菌株显示相同的ST这一事实表明，应开发分辨率更高的其他技术以有效识别感染源。10.7%的菌株对环丙沙星耐药，环丙沙星是推荐用于治疗弓形杆菌肠道感染的抗生素之一，这表明选择最合适的有效治疗方法很重要。
