意大利南部生产的即食蔬菜中 spp. 的发生情况与特征
Occurrence and characterization of spp. from ready-to-eat vegetables produced in Southern Italy.
作者信息
Mottola Anna, Ciccarese Giuseppina, Sinisi Carla, Savarino Alessandra Emilia, Marchetti Patrizia, Terio Valentina, Tantillo Giuseppina, Barrasso Roberta, Di Pinto Angela
机构信息
Department of Veterinary Medicine, University of Bari Aldo Moro, Bari.
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Lecce.
出版信息
Ital J Food Saf. 2021 Mar 25;10(1):8585. doi: 10.4081/ijfs.2021.8585. eCollection 2021 Mar 11.
Abstract
Given that the number of foodborne illness outbreaks linked to the consumption of ready-to-eat vegetables has been widely documented and considering that data on the occurrence of spp. in such foodstuffs are lacking, the aim of the present study was to evaluate the presence of spp. and the occurrence of virulence factors as well as to genotype spp. in ready-to-eat (RTE) vegetable samples, using cultural and biomolecular assays. spp. was detected in 16/110 (14.5%) samples, with being detected in 15/16 and in 1/16 isolates. PCRs aimed at the nine putative virulence genes demonstrated widespread distribution of such genes among and isolates. In addition, multilocus sequence type (MLST) analysis revealed a low genetic diversity within the arcobacters isolates. The results underline the need to develop an appropriate surveillance system based on biomolecular characterization for an integrated microbiological risk assessment of ready-toeat vegetables, and consequently of composite foods.
摘要
鉴于与即食蔬菜消费相关的食源性疾病暴发数量已有大量记录，且考虑到此类食品中特定菌种的发生数据缺乏，本研究的目的是使用培养和生物分子分析方法，评估即食（RTE）蔬菜样品中特定菌种的存在情况、毒力因子的发生情况以及对特定菌种进行基因分型。在110个样品中的16个（14.5%）中检测到了特定菌种，其中15/16的分离株检测到了某菌种，1/16的分离株检测到了另一菌种。针对九个假定毒力基因的PCR表明，这些基因在特定菌种和另一菌种的分离株中广泛分布。此外，多位点序列分型（MLST）分析显示特定弯曲杆菌分离株内的遗传多样性较低。结果强调需要基于生物分子特征开发一个合适的监测系统，以便对即食蔬菜以及复合食品进行综合微生物风险评估。
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