Park Seon Kyeong, Kang Jin Yong, Kim Jong Min, Park Sang Hyun, Kwon Bong Seok, Kim Gun-Hee, Heo Ho Jin
Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Department of Food and Nutrition, Duksung Women's University, Seoul 01369, Republic of Korea.
J Microbiol Biotechnol. 2018 Jan 28;28(1):40-49. doi: 10.4014/jmb.1710.10043.
We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from (FEC) on hydrogen peroxide (H₂O₂)-induced cytotoxicity in PC-12 and MC-IXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C as a positive control for both H₂O₂-treated PC-12 and MC-IXC cells. Based on the neuronal cell-protective effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (Δψm) and ATP levels and regulating mitochondrial-mediated proteins (-AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated (IC₅₀ value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition. Our findings suggest that FEC may be used as a potential material for alleviating oxidative stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.
我们评估了从[具体来源]提取的岩藻依聚糖提取物（FEC）对过氧化氢（H₂O₂）诱导的PC - 12和MC - IXC细胞毒性的抗氧化活性和神经细胞保护作用，以评估其对氧化应激的保护作用。使用ABTS自由基清除活性和丙二醛抑制作用检测抗氧化活性，结果表明FEC具有显著的抗氧化活性。使用DCF - DA检测法和MTT还原检测法研究细胞内活性氧（ROS）含量和神经细胞活力。与作为阳性对照的维生素C相比，FEC对H₂O₂处理的PC - 12和MC - IXC细胞也显示出显著的神经细胞保护作用。基于神经细胞保护作用，在PC - 12细胞中分析线粒体功能，FEC通过增加线粒体膜电位（Δψm）和ATP水平以及调节线粒体介导的蛋白（-AMPK和BAX）显著恢复了线粒体损伤。最后，研究了对乙酰胆碱酯酶（AChE）的抑制作用，乙酰胆碱酯酶是胆碱能系统中神经递质乙酰胆碱的关键水解酶（IC₅₀值 = 1.3 mg/ml），并显示出混合（竞争性和非竞争性）抑制模式。我们的研究结果表明，FEC可能作为一种潜在材料，通过调节线粒体功能和抑制AChE来减轻氧化应激诱导的神经损伤。
