Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Molecules. 2020 Jul 6;25(13):3067. doi: 10.3390/molecules25133067.
Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: , and This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC) values of 2.17 and 49.43 μg/mL, respectively. Most of the tested plant extracts showed higher inhibition of α-glucosidase enzyme activity than the standard, quercetin, particularly , and extracts with IC values of 0.29, 0.36, and 0.51 μg/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)-electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and α-glucosidase inhibitors from .
植物和植物产品长期以来一直被用于药用目的。本研究旨在确定马来西亚八种未充分利用的植物的抗氧化和抗α-葡萄糖苷酶活性： 、 、 、 、 、 和 。研究表明，所有植物的 70%乙醇提取物均表现出总酚含量（TPC）范围为 51 至 344 毫克没食子酸当量（GAE）/克干重。 表现出较强的 2,2-二苯基-1-苦基肼（DPPH）和一氧化氮（NO）清除活性，半数最大抑制浓度（IC）值分别为 2.17 和 49.43 μg/mL。大多数测试的植物提取物对α-葡萄糖苷酶的抑制活性均高于标准品槲皮素，特别是 、 和 提取物的 IC 值分别为 0.29、0.36 和 0.51 μg/mL。通过使用超高效液相色谱（UHPLC）-电喷雾电离（ESI）轨道阱 MS，在最活跃的植物 叶提取物中，共鉴定出 62 种代谢物，包括类黄酮、三萜类、苯醌和脂肪酸。本研究表明 可能是抗氧化剂和α-葡萄糖苷酶抑制剂的天然来源。
