Vicente Ascensión, Ortiz Ruiz Antonio José, García López Miriam, Martínez Beneyto Yolanda, Bravo-González Luis-Alberto
Department of Orthodontics, Faculty of Medicine, University of Murcia, Hospital Morales Meseguer, 2ª planta, C/Marqués de los Vélez s/n, 30008, Murcia, Spain.
Department of Integral Pediatric Dentistry, Faculty of Medicine, University of Murcia, Hospital Morales Meseguer, 2ª planta, C/Marqués de los Vélez s/n, 30008, Murcia, Spain.
Sci Rep. 2017 Nov 9;7(1):15183. doi: 10.1038/s41598-017-15600-5.
The aim of this study was to evaluate the elemental content and morphology of enamel subjected to demineralization cycles after bracket debonding, adhesive remnant removal, and application of a fluoride varnish. 125 bovine teeth were divided into five groups (n = 25): 1) Intact enamel; 2) Intact enamel + demineralization cycles (DC); 3) Enamel after adhesive removal; 4)Enamel after adhesive removal + DC; 5) Enamel after adhesive removal + Profluorid + DC. The weight percentages of calcium (Ca), phosphorous (P) and fluoride (F) were calculated using energy dispersive X-ray spectroscopy (EDX). Samples were observed under scanning electron microscopy (SEM). Data were analyzed using Kruskal-Wallis and Mann-Whitney test. The weight percentages of Ca and P in Group 1 were significantly higher than Groups 2, 4, and 5. The weight percentages of Ca and P in Group 2 were significantly higher than Groups 4 and 5. Group 3 presented significantly higher percentages of Ca and P than Group 4. Group 5 showed a significantly higher percentage of Ca than Group 4. The presence of F was detected in Group 5. SEM images showed more signs of demineralization in Group 4 than Group 5. Fluoride varnish application may protect enamel from demineralization after bracket debonding.
本研究的目的是评估在托槽拆除、去除粘结剂残余物以及应用氟化物清漆后经历脱矿循环的牙釉质的元素含量和形态。125颗牛牙被分为五组(n = 25):1)完整牙釉质;2)完整牙釉质+脱矿循环(DC);3)去除粘结剂后的牙釉质;4)去除粘结剂后的牙釉质+DC;5)去除粘结剂后的牙釉质+Profluorid+DC。使用能量色散X射线光谱法(EDX)计算钙(Ca)、磷(P)和氟(F)的重量百分比。在扫描电子显微镜(SEM)下观察样本。使用Kruskal-Wallis和Mann-Whitney检验分析数据。第1组中Ca和P的重量百分比显著高于第2、4和5组。第2组中Ca和P的重量百分比显著高于第4和5组。第3组中Ca和P的百分比显著高于第4组。第5组中Ca的百分比显著高于第4组。在第5组中检测到F的存在。SEM图像显示第4组中的脱矿迹象比第5组更多。应用氟化物清漆可能会保护牙釉质在托槽拆除后不发生脱矿。