Teruel Juan de Dios, Alcolea Alberto, Hernández Ana, Ruiz Antonio José Ortiz
Department of Integral Pediatric Dentistry, Faculty of Medicine, University of Murcia, Hospital Morales Meseguer, 2ª planta, C/Marqués de los Vélez s/n, 30008 Murcia, Spain.
Servicio de Apoyo a la Investigación Tecnológica, Universidad Politécnica de Cartagena, 30202 Cartagena, Murcia, Spain.
Arch Oral Biol. 2015 May;60(5):768-75. doi: 10.1016/j.archoralbio.2015.01.014. Epub 2015 Feb 20.
The aim of this paper was to compare the chemical composition of human teeth with other mammal species that are likely candidates for replacing them in studies that test dental material.
Dentine and enamel fragments extracted from 400 sound human, bovine, porcine and ovine - 100 teeth per species - incisors and molars were mechanically ground up to a final particle size of less than 100 μm. C/N analysis, thermogravimetric analysis coupled to mass spectrometry (TG-MS), and wavelength dispersive X-ray fluorescence (WDXRF) were used to analyse the samples' composition.
Elemental analysis showed more organic carbon and nitrogen in dentine than in enamel. Human enamel was the most highly mineralised, with C and N values close to hydroxyapatite. Bovine dentine and enamel were the most similar to human. TG-MS: in all species, enamel contained less carbon and organic matter than dentine. Thermal decomposition of human enamel showed great similarity to synthetic hydroxyapatite, and large differences from bovine, ovine and porcine enamel. Thermal decomposition showed the greatest similarity between human and bovine dentine.
Dentine contained larger quantities of Mg, S, Sr and Zn than enamel. Enamel contained larger quantities of P, Ca, Cl, Cu, K and Ca/P ratio than dentine. Human enamel and dentine contained a higher Ca/P ratio, larger quantities of Cl and Cu and lower quantities of Mg, S, Zn than the animal species.
Elemental analysis, TG-MS and WDXRF have shown that human and bovine enamel and dentine show the greatest similarity among the species analysed.
本文旨在比较人类牙齿与其他哺乳动物牙齿的化学成分，这些哺乳动物有可能在牙科材料测试研究中作为人类牙齿的替代物。
从400颗完好的人类、牛、猪和羊的牙齿（每个物种100颗牙齿，包括门牙和臼齿）中提取牙本质和牙釉质碎片，将其机械研磨至最终粒径小于100μm。采用C/N分析、热重分析-质谱联用（TG-MS）和波长色散X射线荧光光谱法（WDXRF）对样品成分进行分析。
元素分析表明，牙本质中的有机碳和氮比牙釉质中的多。人类牙釉质矿化程度最高，碳和氮含量接近羟基磷灰石。牛的牙本质和牙釉质与人类的最为相似。TG-MS分析：在所有物种中，牙釉质中的碳和有机物含量均低于牙本质。人类牙釉质的热分解与合成羟基磷灰石极为相似，与牛、羊和猪的牙釉质有很大差异。热分解显示人类和牛的牙本质之间最为相似。
WDXRF分析：牙本质中的镁、硫、锶和锌含量比牙釉质中的多。牙釉质中的磷、钙、氯、铜、钾含量以及钙/磷比值比牙本质中的多。与动物物种相比，人类牙釉质和牙本质的钙/磷比值更高，氯和铜含量更多，而镁、硫、锌含量更低。
元素分析、TG-MS和WDXRF分析表明，在分析的物种中，人类和牛的牙釉质及牙本质最为相似。
