Pediatric Dentistry Department, Faculty of Dentistry, Ahram Canadian University, Giza, Egypt.
Prosthetic Dentistry Department, Faculty of Dentistry, King Salman International University, El Tur, South Sinai, Egypt.
Eur Arch Paediatr Dent. 2023 Jun;24(3):327-334. doi: 10.1007/s40368-023-00794-2. Epub 2023 Apr 4.
The purpose of this study was to evaluate and compare the remineralization potential of phosphorylated chitosan nanoparticles (Pchi) and silver diamine fluoride (SDF) compared to sodium fluoride varnish (NaF) on microhardness of artificial carious lesions in a biomimetic minimally invasive approach that is being regarded as the future of preventive dentistry.
The sample size included 40 intact extracted maxillary anterior human teeth. Baseline microhardness was recorded using Vickers hardness test and energy-dispersive X-ray spectroscopy (EDX). Artificial caries-like lesions were created on the exposed enamel by suspending all teeth in demineralizing solution for 10 days in a temperature of 37 °C and then the hardness and EDX were remeasured. Samples were then divided into four main groups: Group A (positive control group) n = 10, treated with NaF, Group B n = 10, treated with SDF, Group C n = 10, treated with Pchi and Group D (negative control group) n = 10 that received no treatment. After treatment, samples were incubated in artificial saliva solution at 37 °C in for 10 days and then reassessed. Data were then recorded, tabulated, and statistically analyzed using Kruskal-Wallis test and Wilcoxon signed test. Scanning electron microscope (SEM) was used to analyze the morphological changes of enamel surface after treatment.
Groups B and C showed the highest calcium (Ca) and phosphate (P) content as well as hardness values, while group B had the highest percentage of fluoride. SEM revealed a smooth layer of mineral formed on the surface of enamel for both groups.
Pchi and SDF showed the highest increase in enamel microhardness and remineralization potential.
The minimally invasive approach for remineralization could be enhanced using SDF and Pchi.
本研究旨在评估和比较磷酸化壳聚糖纳米粒子（Pchi）和银胺氟化物（SDF）与氟化钠涂料（NaF）在仿生微创方法中对人工龋损再矿化潜力的影响，这种微创方法被认为是未来预防牙科的发展方向。
样本量包括 40 颗完整的上颌前牙。使用维氏硬度试验和能谱 X 射线光谱（EDX）记录基线微硬度。将所有牙齿悬浮在脱矿溶液中，在 37°C 的温度下放置 10 天，在暴露的牙釉质上产生类似龋齿的病变，然后重新测量硬度和 EDX。然后将样本分为四个主要组：A 组（阳性对照组）n=10，用 NaF 处理；B 组 n=10，用 SDF 处理；C 组 n=10，用 Pchi 处理；D 组（阴性对照组）n=10，不做任何处理。治疗后，将样本在 37°C 的人工唾液溶液中孵育 10 天，然后重新评估。记录、制表和统计分析采用 Kruskal-Wallis 检验和 Wilcoxon 符号秩检验。扫描电子显微镜（SEM）用于分析治疗后牙釉质表面的形态变化。
B 组和 C 组的钙（Ca）和磷（P）含量以及硬度值最高，而 B 组的氟含量最高。SEM 显示，两组牙釉质表面均形成了一层光滑的矿物质层。
Pchi 和 SDF 显示出最高的牙釉质微硬度和再矿化潜力。
微创再矿化方法可以通过使用 SDF 和 Pchi 来增强。
