He Jiaqi, Wang Xuefei, Cai Jianhua, Wang Wei, Qin Xinyu
Department of General Surgery, Huadong Hospital.
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.
Cancer Manag Res. 2017 Oct 25;9:539-544. doi: 10.2147/CMAR.S142324. eCollection 2017.
Eukaryotic initiation factor 3 subunit d (eIF3d) is the largest subunit of eIF3, which is shown to promote protein synthesis in cancer cells. Increased expression of eIF3d has been shown in some types of cancers, but has not been previously studied in gastric cancer (GC). Thus, the aim of this study was to analyze eIF3d expression in GC.
Expression of eIF3d was detected by immunohistochemistry in GC tissues and adjacent noncancerous (ANC) tissues. Samples were obtained from 210 patients with GC who had received curative gastrectomy. Clinicopathological features and survival rate were also analyzed.
Expression rates of eIF3d in GC and ANC were 45.2% and 21.0%, respectively. High expression of eIF3d protein was significantly related to tumor stage, as determined by lymph node metastasis and depth of invasion (<0.05). The Kaplan-Meier survival curves showed that patients with high eIF3d expression had a significantly poor overall survival (=0.005). Multivariate Cox regression analyses showed that the level of eIF3d was an independent predictive factor of poor prognosis for GC (=0.017).
Expression of eIF3d was upregulated in GC. High expression of eIF3d was determined as an independent poor prognostic factor in GC. It is suggested that eIF3d could be a good biomarker in GC.
真核生物起始因子3亚基d（eIF3d）是eIF3的最大亚基，已证明其可促进癌细胞中的蛋白质合成。eIF3d在某些类型的癌症中表达增加，但此前尚未在胃癌（GC）中进行研究。因此，本研究的目的是分析eIF3d在GC中的表达情况。
采用免疫组织化学法检测GC组织及癌旁非癌（ANC）组织中eIF3d的表达。样本取自210例行根治性胃切除术的GC患者。同时分析临床病理特征及生存率。
eIF3d在GC和ANC中的表达率分别为45.2%和21.0%。eIF3d蛋白的高表达与肿瘤分期显著相关，肿瘤分期由淋巴结转移和浸润深度确定（<0.05）。Kaplan-Meier生存曲线显示，eIF3d高表达的患者总生存期显著较差（=0.005）。多因素Cox回归分析表明，eIF3d水平是GC预后不良的独立预测因素（=0.017）。
GC中eIF3d表达上调。eIF3d高表达被确定为GC独立的不良预后因素。提示eIF3d可能是GC的一个良好生物标志物。
