Perić Aleksandar, Mirković Cveta Špadijer, Đurđević Biserka Vukomanović, Perić Aneta V, Vojvodić Danilo
Department of Otorhinolaryngology, Military Medical Academy School of Medicine, Belgrade, Serbia.
Institute for Pathology, Military Medical Academy School of Medicine, Belgrade, Serbia.
Eurasian J Med. 2017 Oct;49(3):178-182. doi: 10.5152/eurasianjmed.2017.17203.
Eotaxin-2 and regulated on activation normal T cell expressed and secreted (RANTES) are involved in the eosinophil trafficking in patients with persistent allergic rhinitis (PAR). Clara cell protein 16 (CC16) is an anti-inflammatory protein mainly produced by the epithelial non-ciliated Clara cells. The aim of this study was to investigate the production of CC16 and chemokines eotaxin-2 and RANTES in nasal mucosa of patients with PAR.
Twenty-one PAR patients and 20 healthy participants were included. CC16, eotaxin-2, and RANTES concentrations were measured in nasal secretions. PAR patients were administered fluticasone furoate nasal spray (220 μg daily for 14 days). We performed nasal cytology, symptom score assessment, and inflammatory mediator detection before and after the therapy.
The level of CC16 in patients with PAR was lower than in the healthy subjects (p=0.023). The eosinophil counts and local concentrations of eotaxin-2 and RANTES were higher in patients with PAR in comparison with controls (p=0.008, p=0.001, p=0.031, respectively). We also found a negative correlation between the CC16 and eotaxin-2 levels in nasal secretions of PAR patients (r=-0.492, p=0.023). After corticosteroid therapy, the patients with PAR had lower nasal symptoms, eosinophil counts, eotaxin-2, and RANTES levels and higher levels of CC16 (p<0.001 for all parameters).
Our results suggest the presence of a negative correlation in production of CC16 and eotaxin-2 in nasal mucosa of patients with PAR. Intranasal corticosteroids have a suppressive effect on mucosal eosinophilic inflammation and a stimulating effect on local CC16 production.
嗜酸性粒细胞趋化因子-2（Eotaxin-2）和活化正常T细胞表达和分泌的调节趋化因子（RANTES）参与持续性变应性鼻炎（PAR）患者的嗜酸性粒细胞迁移。克拉拉细胞蛋白16（CC16）是一种主要由上皮无纤毛克拉拉细胞产生的抗炎蛋白。本研究旨在调查PAR患者鼻黏膜中CC16以及趋化因子Eotaxin-2和RANTES的产生情况。
纳入21例PAR患者和20名健康参与者。测量鼻分泌物中CC16、Eotaxin-2和RANTES的浓度。对PAR患者给予糠酸氟替卡松鼻喷雾剂（每日220μg，共14天）。我们在治疗前后进行了鼻细胞学检查、症状评分评估和炎症介质检测。
PAR患者的CC16水平低于健康受试者（p = 0.023）。与对照组相比，PAR患者的嗜酸性粒细胞计数以及Eotaxin-2和RANTES的局部浓度更高（分别为p = 0.008、p = 0.001、p = 0.031）。我们还发现PAR患者鼻分泌物中CC16和Eotaxin-2水平之间呈负相关（r = -0.492，p = 0.023）。糖皮质激素治疗后，PAR患者的鼻部症状、嗜酸性粒细胞计数、Eotaxin-2和RANTES水平降低，CC16水平升高（所有参数p < 0.001）。
我们的结果表明，PAR患者鼻黏膜中CC16和Eotaxin-2的产生呈负相关。鼻内糖皮质激素对黏膜嗜酸性粒细胞炎症有抑制作用，对局部CC16的产生有刺激作用。
