汉方提取物中β-D-葡聚糖和内毒素水平的测定。
Determination of β-d-glucan and endotoxin levels in Kampo extracts.
作者信息
Nakae Hajime, Takahashi Gaku, Sato Ryo, Shibata Shigehiro, Endo Shigeatsu
机构信息
Department of Emergency and Critical Care Medicine Akita University Graduate School of Medicine Akita Japan.
Department of Critical Care Medicine School of Medicine, Iwate Medical University Morioka Japan.
出版信息
Acute Med Surg. 2014 Oct 1;2(2):77-81. doi: 10.1002/ams2.70. eCollection 2015 Apr.
AIMS
Kampo medicine is based on the use of established formulations combining natural extracts with no "brand-name" products or corresponding "generic" formulation. Due to differences in manufacturing practices, products of different pharmaceutical companies may contain different concentrations of β-d-glucan and endotoxins. The aim of this study was to compare the concentrations of β-d-glucan and endotoxins in five Kampo extracts from four pharmaceutical companies.
METHODS
Packages of Kampo extracts were dissolved in distilled water. β-d-Glucan and endotoxin concentrations were measured using high-sensitivity kinetic turbidimetric Limulus assay.
RESULTS
All Kampo extracts examined in this study were found to contain detectable concentrations of β-d-glucan and endotoxins. Significant differences in the concentration of β-d-glucan and endotoxins ( = 0.0024 and = 0.0013, respectively) were observed between products of different pharmaceutical companies.
CONCLUSIONS
High β-d-glucan and endotoxin contents were detected in Kampo extracts, with a large variability in the concentrations of both β-d-glucan and endotoxins among extracts from different pharmaceutical companies.
目的
汉方医学基于使用既定配方，将天然提取物与无“品牌名称”产品或相应“通用”配方相结合。由于生产工艺的差异，不同制药公司的产品可能含有不同浓度的β - d - 葡聚糖和内毒素。本研究的目的是比较来自四家制药公司的五种汉方提取物中β - d - 葡聚糖和内毒素的浓度。
方法
将汉方提取物包装溶解于蒸馏水中。使用高灵敏度动态比浊法鲎试剂法测量β - d - 葡聚糖和内毒素浓度。
结果
本研究中检测的所有汉方提取物均含有可检测浓度的β - d - 葡聚糖和内毒素。在不同制药公司的产品之间观察到β - d - 葡聚糖和内毒素浓度存在显著差异（分别为= 0.0024和= 0.0013）。
结论
在汉方提取物中检测到高含量的β - d - 葡聚糖和内毒素，不同制药公司提取物中β - d - 葡聚糖和内毒素的浓度存在很大差异。
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