Braden Jessica L, Klarquist Emily F, Kellogg Julianne A
Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA.
Food Chem X. 2024 Sep 17;24:101844. doi: 10.1016/j.fochx.2024.101844. eCollection 2024 Dec 30.
A novel method for multi-element analysis in cereals, pseudocereals, and legumes was developed for principal (calcium, magnesium, potassium, and phosphorus) and trace (manganese, zinc, iron, copper, and aluminum) element determination using a microwave plasma-atomic emission spectrometer (MP-AES). The method was validated using certified reference analyte values from durum wheat (DUWF-1), corn bran (BRAN-1), quinoa (KINO-1), rice (SRM 1568b), and soy (SRM 3234). Spike recoveries were assessed using field-grown crops that represent staple and minor crops with variable matrix compositions. A closed-vessel microwave-assisted digestion method consisting of 12 mL of deionized water, 2 mL of HNO3, and 2 mL of H2O2 was efficient for the mineralization of all crops. Acceptable measurement agreement was achieved between certified and determined values for all reference materials with recovery ranges from 89 to 120 percent. Plant breeders can use the method to develop and screen crops for improved nutrient density.
开发了一种用于谷物、伪谷物和豆类中多元素分析的新方法，该方法使用微波等离子体原子发射光谱仪（MP-AES）测定主要元素（钙、镁、钾和磷）和微量元素（锰、锌、铁、铜和铝）。该方法通过使用硬粒小麦（DUWF-1）、玉米麸皮（BRAN-1）、藜麦（KINO-1）、大米（SRM 1568b）和大豆（SRM 3234）的认证参考分析物值进行了验证。使用代表具有可变基质组成的主粮和次要作物的田间种植作物评估加标回收率。由12 mL去离子水、2 mL HNO3和2 mL H2O2组成的密闭容器微波辅助消解方法对所有作物的矿化有效。所有参考物质的认证值和测定值之间均达成了可接受的测量一致性，回收率范围为89%至120%。植物育种者可以使用该方法来开发和筛选具有更高营养密度的作物。
