利用DNA四面体构建圆形组件：从静态结构到动态旋转马达
Fabrication of circular assemblies with DNA tetrahedrons: from static structures to a dynamic rotary motor.
作者信息
Wang Liying, Meng Zhenyu, Martina Felicia, Shao Huilin, Shao Fangwei
机构信息
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371 Singapore.
Biomedical Institute of Global Heath Research and Technology, Departments of Biomedical Engineering and Surgery, National University of Singapore, Singapore.
出版信息
Nucleic Acids Res. 2017 Dec 1;45(21):12090-12099. doi: 10.1093/nar/gkx1045.
DNA tetrahedron as the simplest 3D DNA nanostructure has been applied widely in biomedicine and biosensing. Herein, we design and fabricate a series of circular assemblies of DNA tetrahedron with high purity and decent yields. These circular nanostructures are confirmed by endonuclease digestion, gel electrophoresis and atomic force microscopy. Inspired by rotary protein motor, we demonstrate these circular architectures can serve as a stator for a rotary DNA motor to achieve the circular rotation. The DNA motor can rotate on the stators for several cycles, and the locomotion of the motor is monitored by the real-time fluorescent measurements.
DNA四面体作为最简单的三维DNA纳米结构，已在生物医学和生物传感领域得到广泛应用。在此，我们设计并制备了一系列具有高纯度和良好产率的DNA四面体圆形组装体。这些圆形纳米结构通过核酸内切酶消化、凝胶电泳和原子力显微镜得到证实。受旋转蛋白马达的启发，我们证明这些圆形结构可以作为旋转DNA马达的定子来实现圆周旋转。DNA马达可以在定子上旋转几个周期，并且通过实时荧光测量来监测马达的运动。
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