Yang Yangyang, Tashiro Ryu, Suzuki Yuki, Emura Tomoko, Hidaka Kumi, Sugiyama Hiroshi, Endo Masayuki
Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto, 606-8501, Japan.
Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Chemistry. 2017 Mar 17;23(16):3979-3985. doi: 10.1002/chem.201605616. Epub 2017 Feb 15.
Various DNA-based nanodevices have been developed on the nanometer scale using light as regulation input. However, the programmed controllability is still a major challenge for these artificial nanodevices. Herein, we demonstrate a rotary DNA nanostructure in which the rotations are controlled by light. A bar-shaped DNA rotor, fabricated as a stiff double-crossover molecule, was placed on the top of a rectangular DNA tile. The photoresponsive oligonucleotides modified with azobenzenes were employed as switching motifs to release/trap the rotor at specific angular position on DNA tile by switching photoirradiations between ultraviolet and visible light. As a result, two reconfigurable states (perpendicular and parallel) of rotor were obtained, in which the angular changes were characterized by AFM and fluorescence quenching assays. Moreover, the reversible rotary motions during the photoirradiation were directly visualized on the DNA tile surface in a nanometer-scale precision using a second-scale scanning of the high-speed AFM.
各种基于DNA的纳米器件已在纳米尺度上利用光作为调控输入进行了开发。然而，对于这些人工纳米器件而言，编程可控性仍然是一个重大挑战。在此，我们展示了一种旋转DNA纳米结构，其中的旋转由光控制。一个制成刚性双交叉分子的棒状DNA转子被放置在一个矩形DNA瓦片的顶部。用偶氮苯修饰的光响应寡核苷酸被用作开关基序，通过在紫外光和可见光之间切换光照射，在DNA瓦片上的特定角度位置释放/捕获转子。结果，获得了转子的两种可重构状态（垂直和平行），其中角度变化通过原子力显微镜（AFM）和荧光猝灭测定进行表征。此外，利用高速AFM的二级扫描，在纳米尺度精度下直接在DNA瓦片表面可视化了光照射期间的可逆旋转运动。
