Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29, Geumgu-gil, Jeongup, Jeonbuk 56212, Republic of Korea.
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29, Geumgu-gil, Jeongup, Jeonbuk 56212, Republic of Korea; Department of Food Science and Technology, Kongju National University, 54, Daehak-ro, Yesan, Chungnam 32439, Republic of Korea.
Food Chem Toxicol. 2018 Jan;111:133-143. doi: 10.1016/j.fct.2017.11.006. Epub 2017 Nov 7.
Gamma irradiation is able to affect various structural modification and an increase of the biological properties of biomaterials. This study was conducted to investigate the anti-allergenic effect of γ-irradiated black ginseng extract (BGE) using in vitro and in vivo experiments. IgEantigen complex-induced degranulation was measured in RBL-2H3 mast cells. In addition, an anti-atopic dermatitis (AD) test was carried out by spreading γ-irradiated BGE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice. The content of arginylfructose (AF) of gamma-irradiated BGE was higher than that of BGE. In RBL-2H3 mast cells, γ-irradiated BGE treatments significantly reduced the IgE-antigen complex-induced release of β-hexosaminidase, histamine, intracellular ROS, and Ca2+ influx. A western blot analysis showed that γ-irradiated BGE had an inhibitory activity on the FcεRI-mediated signaling in mast cells. In the DNCB-induced AD model, γ-irradiated BGE significantly alleviated the ADlike skin symptoms and clinical signs. The suppression of AD by γ-irradiated BGE was accompanied by a decrease in the serum level of IgE and IL-4, as well as the number of leukocyte. Gamma-irradiated BGE also suppressed IL-4 and increased IFN-γ in splenocytes. Our data suggests that γ-irradiated BGE may be effective therapeutic agents for the treatment of AD.
γ 射线辐照能够影响生物材料的各种结构修饰和生物性能的提高。本研究通过体外和体内实验研究了 γ 射线辐照黑参提取物（BGE）的抗过敏原作用。在 RBL-2H3 肥大细胞中测量 IgE 抗原复合物诱导的脱颗粒。此外，通过将 γ 射线辐照的 BGE 涂抹在 2,4-二硝基氯苯（DNCB）诱导的 BALB/c 小鼠的背部皮肤上进行了抗特应性皮炎（AD）测试。γ 射线辐照的 BGE 的精氨酰果糖（AF）含量高于 BGE。在 RBL-2H3 肥大细胞中，γ 射线辐照的 BGE 处理显著降低了 IgE 抗原复合物诱导的β-己糖胺酶、组胺、细胞内 ROS 和 Ca2+内流的释放。Western blot 分析表明，γ 射线辐照的 BGE 对肥大细胞中 FcεRI 介导的信号具有抑制活性。在 DNCB 诱导的 AD 模型中，γ 射线辐照的 BGE 显著缓解了 AD 样皮肤症状和临床体征。γ 射线辐照的 BGE 抑制 AD 的同时伴随着血清 IgE 和 IL-4 水平以及白细胞数量的降低。γ 射线辐照的 BGE 还抑制了脾细胞中的 IL-4 并增加了 IFN-γ。我们的数据表明，γ 射线辐照的 BGE 可能是治疗 AD 的有效治疗剂。
