Department of Plant Pathology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt.
Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt.
Microb Pathog. 2018 Aug;121:331-340. doi: 10.1016/j.micpath.2018.05.032. Epub 2018 May 23.
Three bacterial isolates were identified from infected potato tubers showing soft and blackleg like symptoms as well as one isolate from infected pear tree showing crown gall symptom. Conventional and molecular identification proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum, Dickeya solani and Agrobacterium tumefaciens. The above plant bacterial isolates and human pathogenic bacteria Escherichia coli, Sarcina lutea, and Staphylococcus aureus were used for the bioassay. The chloroform leaf extracts from Duranta plumieri variegata, Lantana camara, and Citharexylum spinosum were assayed for their antibacterial activity by measuring the inhibition zones and minimum inhibitory concentrations (MICs). The suggested chemical compositions of extracts were analyzed using GC/MS apparatus. The main compounds in leaf extract of L. camara were 5,8-diethyl-dodecane, pyrimidin-2-one, 4-[N-methylureido]-1-[4-methylaminocarbonyloxymethyl, oleic acid,3-(octadecyloxy)propyl ester; in D. plumieri were 4,7-dimethoxy-2-methylindan-1-one and 5-(hexadecyloxy)-2-pentadecyl-,trans-1,3-dioxane; and in C. spinosum were N-[5-(3-hydroxy-2-methylpropenyl)-1,3,4,5-tetrahydrobenzo[cd]indol-3-yl]-N-methylacetamide. Promising activity was found against A. tumefaciens, E. coli, P. carotovorum, Sar. lutea, and Staph. aureus with MIC values of 8, 128, 64, 500 and 500 μg/mL, respectively, as L. camara leaf extract was applied. D. plumieri leaf extract showed good activity against D. solani and P. atrosepticum with MIC values of 16 μg/mL and 128 μg/mL, respectively. On the other hand, weak bioactivity was found with leaf extract from C. spinosum. It could be concluded that leaf extracts from D. plumieri and L. camara have a promising antibacterial agents.
从表现软腐和黑胫病症状的感染马铃薯块茎中分离出 3 株细菌分离株，以及一株表现冠瘿病症状的感染梨树的分离株。常规和分子鉴定证明，细菌分离株属于胡萝卜软腐果胶杆菌亚种、果胶杆菌、软腐病棒杆菌和根癌农杆菌。将上述植物细菌分离株和人类病原菌大肠杆菌、藤黄微球菌和金黄色葡萄球菌用于生物测定。用氯仿提取 Duranta plumieri variegata、Lantana camara 和 Citharexylum spinosum 的叶片提取物，并通过测量抑菌圈和最小抑菌浓度（MIC）来测定其抗菌活性。使用 GC/MS 仪器分析提取物的建议化学成分。L. camara 叶片提取物中的主要化合物为 5,8-二乙基-十二烷、嘧啶-2-酮、4-[N-甲基脲基]-1-[4-甲基氨基羰基氧甲基、油酸 3-（十八烷氧基）丙酯；D. plumieri 中为 4,7-二甲氧基-2-甲基茚满-1-酮和 5-（十六烷氧基）-2-十五烷基-，反式-1,3-二氧戊环；C. spinosum 中为 N-[5-（3-羟基-2-甲基丙烯基）-1,3,4,5-四氢苯并[cd]吲哚-3-基]-N-甲基乙酰胺。用 L. camara 叶提取物处理时，对根癌农杆菌、大肠杆菌、胡萝卜软腐果胶杆菌、藤黄微球菌和金黄色葡萄球菌表现出有希望的活性，MIC 值分别为 8、128、64、500 和 500μg/mL。D. plumieri 叶提取物对 D. solani 和 P. atrosepticum 表现出良好的活性，MIC 值分别为 16μg/mL 和 128μg/mL。另一方面，C. spinosum 叶提取物的生物活性较弱。可以得出结论，D. plumieri 和 L. camara 的叶提取物具有有前途的抗菌剂。
