The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510275, and Key Laboratory of Molecular Clinical Pharmacology, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, People's Republic of China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
Sci Rep. 2017 Nov 10;7(1):15250. doi: 10.1038/s41598-017-15368-8.
Hemostasis in vivo is a key to success in minimally invasive surgery (MIS). However, solid hemostatic materials cannot pass through the sheath tube of the MIS apparatus, while liquid ones are restricted by their low adhesion, which leads to them peeling off of tissue. To tackle the dilemma of fluidity and adhesion, a formulation containing a multifunctional sucrose allyl ether (SAE) monomer and an alpha-hydroxyketone liquid photoinitiator (HMPP) was applied as a lead hemostatic material for MIS. Real-time infrared results showed that SAE initiated by HMPP can rapidly polymerize into a transparent crosslinking membrane. Quantum chemistry showed that this occurs via a free radical addition reaction mechanism. Thermodynamic properties, such as reaction driving force and enthalpy change, were similar to those for a corresponding small molecular analogue, allyl methyl ether (AME), but the addition rate was lower than that for AME. The CC50 values of SAE and HMPP were also obtained by cell experiments. A hemostasis experiment in vivo was performed by comparing the formulation with chitosan and a traditional Chinese medicine (Yunnan Baiyao powder). The result showed that the formulation had a competitive advantage for use in MIS.
体内止血是微创外科（MIS）成功的关键。然而，固体止血材料无法通过 MIS 仪器的鞘管，而液体材料则受到其低附着力的限制，这导致它们从组织上剥落。为了解决流动性和附着力的困境，一种含有多功能蔗糖烯丙基醚（SAE）单体和α-羟基酮液体光引发剂（HMPP）的配方被用作 MIS 的主要止血材料。实时红外结果表明，HMPP 引发的 SAE 可以迅速聚合形成透明的交联膜。量子化学表明，这是通过自由基加成反应机制发生的。热力学性质，如反应驱动力和焓变，与相应的小分子类似物烯丙基甲醚（AME）相似，但加成速率低于 AME。通过细胞实验还获得了 SAE 和 HMPP 的 CC50 值。通过比较该配方与壳聚糖和一种中药（云南白药粉）进行体内止血实验。结果表明，该配方在 MIS 中具有竞争优势。
