Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Department of Veterinary Pathology, Rakuno Gakuen University, Hokkaido, Japan.
Virology. 2018 Jan 15;514:57-65. doi: 10.1016/j.virol.2017.10.021. Epub 2018 Jan 4.
Pteropine orthoreovirus (PRV) is an emerging bat-borne human pathogen causing severe respiratory illness. To date, however, the evaluation of PRV virulence has largely depended on the limited numbers of clinical cases owing to the lack of animal models. To develop an in vivo model of PRV infection, an inbred C3H mouse strain was infected intranasally with pathogenic PRV strain Miyazaki-Bali/2007. C3H mice suffered severe lung infection with significant body weight reduction and died within 7 days after intranasal infection. Infectious viruses were isolated mainly from the lungs and trachea. Histopathological examination revealed interstitial pneumonia with monocytes infiltration. Following repeated intranasal infection, mice developed antibodies to particular structural and non-structural proteins of PRV. The results of these immunological assays will help to develop laboratory protocols for sero-epidemiological studies. Our small rodent model of lethal respiratory infection will further allow investigation of the molecular mechanisms underlying the high pathogenicity of PRV.
果蝠正呼肠孤病毒（PRV）是一种新兴的蝙蝠传播的人类病原体，可引起严重的呼吸道疾病。然而，由于缺乏动物模型，迄今为止，对 PRV 毒力的评估在很大程度上取决于有限的临床病例数量。为了建立 PRV 感染的体内模型，用致病性 PRV 株 Miyazaki-Bali/2007 经鼻腔感染近交系 C3H 小鼠。C3H 小鼠肺部严重感染，体重明显减轻，鼻腔感染后 7 天内死亡。从肺部和气管中主要分离到传染性病毒。组织病理学检查显示间质性肺炎伴单核细胞浸润。经多次鼻腔感染后，小鼠产生针对 PRV 特定结构和非结构蛋白的抗体。这些免疫学检测的结果将有助于制定血清流行病学研究的实验室方案。我们的致死性呼吸道感染小型啮齿动物模型将进一步允许研究 PRV 高致病性的分子机制。
