Universidade de Sao Paulo, Ribeirão Preto, São Paulo, Brazil.
J Bioenerg Biomembr. 2017 Dec;49(6):423-435. doi: 10.1007/s10863-017-9732-x. Epub 2017 Nov 11.
Our understanding of nicotinamide adenine dinucleotide mitochondrial transporter 1 (Ndt1A) in Aspergillus fumigatus remains poor. Thus, we investigated whether Ndt1A could alter fungi survival. To this end, we engineered the expression of an Ndt1A-encoding region in a Δndt1Δndt2 yeast strain. The resulting cloned Ndt1A protein promoted the mitochondrial uptake of nicotinamide adenine dinucleotide (NAD), generating a large mitochondrial membrane potential. The NAD carrier utilized the electrochemical proton gradient to drive NAD entrance into mitochondria when the mitochondrial membrane potential was sustained by succinate. Its uptake has no impact on oxidative stress, and Ndt1A expression improved growth and survival of the Δndt1Δndt2 Saccharomyces cerevisiae strain.
我们对烟曲霉烟酰胺腺嘌呤二核苷酸线粒体转运蛋白 1（Ndt1A）的了解仍然有限。因此，我们研究了 Ndt1A 是否可以改变真菌的生存能力。为此，我们在Δndt1Δndt2 酵母菌株中设计了 Ndt1A 编码区域的表达。所得克隆的 Ndt1A 蛋白促进了烟酰胺腺嘌呤二核苷酸（NAD）的线粒体摄取，产生了大的线粒体膜电位。当线粒体膜电位被琥珀酸维持时，NAD 载体利用电化学质子梯度驱动 NAD 进入线粒体。其摄取对氧化应激没有影响，并且 Ndt1A 的表达改善了 Δndt1Δndt2 酿酒酵母菌株的生长和存活。
