Department of Wildlife Ecology & Management, Karadeniz Technical University Faculty of Forestry, 61080, Trabzon, Turkey.
Fisheries Faculty, Munzur University, 62000, Tunceli, Turkey.
Environ Sci Pollut Res Int. 2018 Jan;25(3):2588-2593. doi: 10.1007/s11356-017-0700-0. Epub 2017 Nov 11.
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
随着农业活动的增加，农药的使用量也有所增加，这对环境造成了有害的影响。特别是包括鱼类在内的非目标生物可能会受到农药的毒性影响。因此，本研究在体外研究了马拉硫磷（MTN）对氧化应激和精子质量的影响。本研究使用的 MTN 浓度分别为 0（对照）、75、100 和 125μg/L。检测精子中的脂质过氧化（MDA）、非酶（GSH）和酶（SOD、GSH-Px 和 CAT）活性，以确定氧化应激状态。我们的研究结果表明，随着 MTN 的暴露，精子的运动率和活动期显著下降。生化分析显示，基于浓度，CAT 活性和 MDA、GSH 的水平增加，而 GSH-Px 和 SOD 的活性降低。因此，精子对 MTN 暴露非常敏感。MTN 对精子质量有破坏作用，并导致精子中的氧化应激。
