Kutluyer Filiz, Benzer Fulya, Erişir Mine, Öğretmen Fatih, İnanan Burak Evren
Tunceli University, Fisheries Faculty, 62000, Tunceli, Turkey.
Tunceli University, Faculty of Engineering, Department of Food Engineering, 62000, Tunceli, Turkey.
Pestic Biochem Physiol. 2016 Mar;128:63-7. doi: 10.1016/j.pestbp.2015.10.001. Epub 2015 Oct 8.
There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 μg/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P<0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P<0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin.
关于鱼类精子体外暴露于农药的影响，科学文献中的信息有限。氯氰菊酯对精子质量和氧化应激的体外影响尚未得到充分研究。因此，在体外研究了II型拟除虫菊酯类杀虫剂氯氰菊酯对精子质量和氧化应激的影响。为了探索氯氰菊酯潜在的体外毒性，将鱼类精子与不同浓度的氯氰菊酯（1.025、2.05和4.1μg/l）孵育2小时。在暴露于氯氰菊酯后测定精子的活力率和持续时间。分析精子中的还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和丙二醛（MDA），以确定氧化剂和抗氧化剂的平衡。我们的结果表明，暴露于氯氰菊酯后精子活力和持续时间显著降低。此外，GSH-Px的活性（P<0.05）以及MDA和GSH水平呈浓度依赖性增加，而CAT活性降低（P<0.05）。因此，氧化剂和抗氧化剂状态以及精子质量受到氯氰菊酯定量变化和不同浓度的影响。
