Gynecol Obstet Invest. 2018;83(3):268-274. doi: 10.1159/000484243. Epub 2017 Nov 1.
BACKGROUND/AIMS: Survival of warmed oocytes is the first part and is one of the most important aspects of in vitro fertilization following oocyte cryopreservation. There is no definite conclusion about the difference in survival rates between closed and open vitrification for oocyte cryopreservation. This study aimed to investigate whether there is a difference in survival rates between closed and open vitrification for cryopreservation of human mature oocytes.
A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases. A total of 4 studies were included in the meta-analysis.
In the frequentist meta-analysis, the random effects model showed no statistically significant difference in survival rates between closed and open vitrification (risk ratio [RR] 0.8873, 95% confidence interval [CI] 0.7658-1.0280). However, an effect size deviation of the RR from 1.0 and a borderline trend of the 95% CI were noted. In the Bayesian meta-analysis, the posterior probability that closed vitrification would decrease the survival rate when compared to that with open vitrification was 83.04% for the random effects model.
It is not yet possible to conclude that closed vitrification is an aseptic alternative to open vitrification in human mature oocyte cryopreservation.
背景/目的：解冻卵母细胞的存活率是体外受精的第一步，也是卵母细胞冷冻保存后最重要的方面之一。对于卵母细胞冷冻保存的封闭法和开放法玻璃化，哪种方法能更有效地提高卵母细胞的存活率还没有明确的结论。本研究旨在探讨人成熟卵母细胞冷冻保存的封闭法和开放法玻璃化在存活率方面是否存在差异。
使用 MEDLINE、Cochrane 对照试验中心注册库和 Embase 数据库进行文献检索。共有 4 项研究纳入荟萃分析。
在经典概率法荟萃分析中，随机效应模型显示封闭法和开放法玻璃化在存活率方面无统计学差异（风险比[RR]0.8873，95%置信区间[CI]0.7658-1.0280）。然而，RR 值的效应量偏离 1.0，95%CI 呈边界趋势。在贝叶斯荟萃分析中，对于随机效应模型，封闭法玻璃化降低存活率的后验概率为 83.04%。
目前还不能得出结论认为在人成熟卵母细胞冷冻保存中，封闭法玻璃化是开放法玻璃化的无菌替代方法。
