Eng Chee-Huat Linus, Shah Sheel, Thomassie Julian, Cai Long
Division of Chemistry and Chemical Engineering, Caltech, Pasadena, CA, USA.
UCLA-Caltech Medical Scientist Training Program, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA, USA.
Nat Methods. 2017 Dec;14(12):1153-1155. doi: 10.1038/nmeth.4500. Epub 2017 Nov 13.
Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential probing of targets (SPOTs), provides an accurate, flexible, and low-cost alternative to sequencing for profiling transcriptomes.
单分子荧光原位杂交（smFISH）一直是定量单个转录本丰度的金标准。在此，我们将多重smFISH扩展到转录组水平，并对来自小鼠成纤维细胞和胚胎干细胞的10212种不同mRNA进行分析。这种方法称为RNA靶标序列探测（SPOTs），为转录组分析提供了一种准确、灵活且低成本的测序替代方法。
