Souza S L, Graça G, Oliva A
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Skin Res Technol. 2018 May;24(2):187-195. doi: 10.1111/srt.12412. Epub 2017 Nov 12.
BACKGROUND/PURPOSE: The elimination of the pain associated with needle picking is a strong motivation for the development of clinical non-invasive diagnostic methods. Sweat has been described as an alternative biological sample that may have a direct relation to the plasma composition.
In this study, analysis of sweat of human volunteers obtained by induction with pilocarpine is compared with sweat samples obtained by physical exercise and by passive collection along 7 hours. The sweat samples have been analyzed by 1H nuclear magnetic resonance spectroscopy.
A range of 34 different metabolites has been detected in sweat samples, including lactate, several amino acids, pyroglutamate, and urocanate. Most of the metabolites identified were quantified. The majority of the amino acids detected in sweat seem to have origin in the epidermis surface. No significant differences in sweat samples from female and male were observed by 1H NMR metabolomic analysis.
Principal component analysis (PCA) shows that both physical exercise and pilocarpine methods seem to be equally reproducible methods in terms of sweat metabolite composition presenting better repeatability than natural sweat collection. Nevertheless, this difference is mainly originated from amino acids with origin from the skin surface.
背景/目的：消除与针刺采血相关的疼痛是开发临床非侵入性诊断方法的强大动力。汗液已被描述为一种可能与血浆成分有直接关系的替代生物样本。
在本研究中，将通过毛果芸香碱诱导获得的人类志愿者汗液分析结果与通过体育锻炼以及7小时被动收集获得的汗液样本进行比较。汗液样本采用1H核磁共振波谱法进行分析。
在汗液样本中检测到34种不同的代谢物，包括乳酸、几种氨基酸、焦谷氨酸和尿刊酸。鉴定出的大多数代谢物都进行了定量分析。汗液中检测到的大多数氨基酸似乎起源于表皮表面。通过1H NMR代谢组学分析，未观察到女性和男性汗液样本之间存在显著差异。
主成分分析（PCA）表明，就汗液代谢物组成而言，体育锻炼和毛果芸香碱方法似乎都是同样可重复的方法，其重复性优于自然汗液收集。然而，这种差异主要源于来自皮肤表面的氨基酸。
