Fayad Syntia, Morin Philippe, Nehmé Reine
Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans - CNRS, UMR 7311, Orléans, France.
Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans - CNRS, UMR 7311, Orléans, France.
J Chromatogr A. 2017 Dec 22;1529:1-28. doi: 10.1016/j.chroma.2017.11.003. Epub 2017 Nov 9.
Elastase, collagenase, hyaluronidase and tyrosinase, are very interesting enzymes due to their direct implication in skin aging and as therapeutic hits. Different techniques can be used to study these enzymes and to evaluate the influence of effectors on their kinetics. Nowadays, analytical techniques have become frequently used tools for miniaturizing enzyme assays. The main intention of this article is to review chromatographic and electrophoretic tools that study the four enzymes above mentioned. More specifically, the use of high-performance liquid chromatography and capillary electrophoresis and their derivative techniques for monitoring these enzymes will be investigated. The advantages and limitations of these assays will also be discussed. The original use of microscale thermophoresis and thin layer chromatography in this domain will also be covered.
弹性蛋白酶、胶原酶、透明质酸酶和酪氨酸酶是非常有趣的酶，因为它们直接参与皮肤老化过程，并且是治疗靶点。可以使用不同的技术来研究这些酶，并评估效应物对其动力学的影响。如今，分析技术已成为酶分析小型化常用的工具。本文的主要目的是综述用于研究上述四种酶的色谱和电泳工具。更具体地说，将研究使用高效液相色谱和毛细管电泳及其衍生技术来监测这些酶。还将讨论这些分析方法的优缺点。本文还将涵盖微量热泳动和薄层色谱在该领域的最初应用。
