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一种来自 Brenneria sp. EniD312 的高分子量槐糖胶的理化性质。

Physicochemical properties of a high molecular weight levan from Brenneria sp. EniD312.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, People's Republic of China.

出版信息

Int J Biol Macromol. 2018 Apr 1;109:810-818. doi: 10.1016/j.ijbiomac.2017.11.056. Epub 2017 Nov 11.

DOI:10.1016/j.ijbiomac.2017.11.056
PMID:29133100
Abstract

A high molecular weight levan was produced by a novel levansucrase and some properties of this polymer were investigated. The levan exhibited a poroid microstructure as well as series of individual ellipsoidal or spheroidal particles. The weight-average molecular weight (M¯) of the levan was determined to be 1.41×10Da. In a 0.1% solution, the levan showed a mean diameter of 176nm, while in a 1% solution the diameter was 182nm. The decomposition temperature was determined to be 216.67°C, with an endothermic peak at 147.41°C and a melting enthalpy of 76.9J/g. The small angle X-ray diffraction pattern showed a distinctive peak pattern between 15° and 40° (2q). The levan solution showed a shear-thinning behaviour. These results suggest this levan could be a good additive in the food processing industry, as well as an important bio-based material in the medicinal or chemical industry.

摘要

一种新型的蔗聚糖蔗糖酶可产生高分子量的蔗聚糖,且对该聚合物的一些性质进行了研究。该蔗聚糖呈现多孔微观结构,以及一系列单独的椭圆形或球形颗粒。蔗聚糖的重均分子量(M¯)确定为 1.41×10Da。在 0.1%的溶液中,蔗聚糖的平均直径为 176nm,而在 1%的溶液中直径为 182nm。分解温度确定为 216.67°C,在 147.41°C 处有一个吸热峰,熔融焓为 76.9J/g。小角度 X 射线衍射图谱在 15°至 40°(2q)之间显示出独特的峰型图案。蔗聚糖溶液表现出剪切稀化行为。这些结果表明,这种蔗聚糖可以成为食品加工行业的一种良好添加剂,以及在医药或化学工业中的一种重要的基于生物的材料。

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