Phengnoi Pongtorn, Teerakulkittipong Nuttinee, Teeparuksapun Kosin, Lirio Gary Antonio, Jangiam Witawat
Department of Chemical Engineering, Faculty of Engineering, Burapha University, Saensuk, Mueang, Chonburi 20131, Thailand.
Faculty of Pharmaceutical Sciences, Burapha University, Saensuk, Mueang, Chonburi 20131, Thailand.
Biotechnol Rep (Amst). 2025 Jul 29;47:e00908. doi: 10.1016/j.btre.2025.e00908. eCollection 2025 Sep.
Levan, a promising fructan polysaccharide for biopharmaceuticals, has limited large-scale production studies. This research optimized and scaled up levan biosynthesis from in continuous stirred-tank bioreactors based on response surface methodology (RSM). Batch cultures optimized for 30 % (w/v) sucrose, pH 5.0, and 48 h incubation yielded a maximum 15.74 % (w/v) levan. The optimal batch conditions were evaluated in a continuous stirred-tank bioreactor, where dilution rates and mixing speeds were examined. At a dilution rate of 0.021 h⁻¹ and an agitation speed of 200 rpm, the maximum productivity was 17.96 % (w/v), and steady-state conditions were attained after three days of continuous fermentation. X-ray diffraction confirmed the amorphous nature of the levan, ideal for biomaterial applications. These results underline the potential of for high-yield levan production and provide a systematic approach for bioprocess parameter optimization, serving as a strong basis for its increased application in industrialized polysaccharide-based bioprocessing.
果聚糖是一种很有前景的用于生物制药的多糖，但大规模生产研究有限。本研究基于响应面法（RSM）在连续搅拌罐式生物反应器中对果聚糖的生物合成进行了优化和放大。优化后的分批培养条件为30%（w/v）蔗糖、pH 5.0和48小时培养，果聚糖产量最高可达15.74%（w/v）。在连续搅拌罐式生物反应器中评估了最佳分批培养条件，并考察了稀释率和混合速度。在稀释率为0.021 h⁻¹和搅拌速度为200 rpm的条件下，最大生产率为17.96%（w/v），连续发酵三天后达到稳态条件。X射线衍射证实了果聚糖的无定形性质，这对于生物材料应用来说是理想的。这些结果突出了[具体内容缺失]用于高产果聚糖生产的潜力，并为生物工艺参数优化提供了一种系统方法，为其在工业化多糖基生物加工中的更多应用奠定坚实基础。
