Wang Jingzhen, Hu Ping, Gao Peng, Tao Jing, Luo Youqing
Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083, P. R. China.
Sci Rep. 2017 Nov 13;7(1):15470. doi: 10.1038/s41598-017-15425-2.
Olfaction in insects is essential for host identification, mating and oviposition, in which olfactory proteins are responsible for chemical signaling. Here, we determined the transcriptomes of male and female adult antennae of Anoplophora chinensis, the citrus longhorned beetle. Among 59,357 unigenes in the antennal assembly, we identified 46 odorant-binding proteins, 16 chemosensory proteins (CSPs), 44 odorant receptors, 19 gustatory receptors, 23 ionotropic receptors, and 3 sensory neuron membrane proteins. Among CSPs, AchiCSP10 was predominantly expressed in antennae (compared with legs or maxillary palps), at a significantly higher level in males than in females, suggesting that AchiCSP10 has a role in reception of female sex pheromones. Many highly expressed genes encoding CSPs are orthologue genes of A. chinensis and Anoplophora glabripennis. Notably, AchiPBP1 and AchiPBP2 showed 100% and 96% identity with AglaPBP1 and AglaPBP2 from A. glabripennis, with similar expression profiles in the two species; PBP2 was highly expressed in male antennae, whereas PBP1 was expressed in all three tissues in both males and females. These results provide a basis for further studies on the molecular chemoreception mechanisms of A. chinensis, and suggest novel targets for control of A. chinensis.
嗅觉对于昆虫识别宿主、交配和产卵至关重要，其中嗅觉蛋白负责化学信号传导。在此，我们测定了柑橘长卷叶蛾成虫雌雄触角的转录组。在触角组装体的59357个单基因中，我们鉴定出46种气味结合蛋白、16种化学感受蛋白（CSPs）、44种气味受体、19种味觉受体、23种离子型受体和3种感觉神经元膜蛋白。在CSPs中，AchiCSP10主要在触角中表达（与腿部或下颚须相比），在雄性中的表达水平显著高于雌性，这表明AchiCSP10在雌性性信息素的接收中起作用。许多编码CSPs的高表达基因是柑橘长卷叶蛾和光肩星天牛的直系同源基因。值得注意的是，AchiPBP1和AchiPBP2与光肩星天牛的AglaPBP1和AglaPBP2的一致性分别为100%和96%，在两个物种中的表达谱相似；PBP2在雄性触角中高表达，而PBP1在雄性和雌性的所有三种组织中均有表达。这些结果为进一步研究柑橘长卷叶蛾的分子化学感受机制提供了基础，并为柑橘长卷叶蛾的防治提出了新的靶点。
