Wang Ran, Li Fengqi, Zhang Wei, Zhang Xiaoman, Qu Cheng, Tetreau Guillaume, Sun Lujuan, Luo Chen, Zhou Jingjiang
Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Laboratoire Intéraction Hôtes-Pathogènes-Environnement, Université de Perpignan, Perpignan, France.
PLoS One. 2017 Feb 6;12(2):e0171739. doi: 10.1371/journal.pone.0171739. eCollection 2017.
Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) of arthropods are thought to be involved in chemical recognition which regulates pivotal behaviors including host choice, copulation and reproduction. In insects, OBPs and CSPs located mainly in the antenna but they have not been systematically characterized yet in Bemisia tabaci which is a cryptic species complex and could damage more than 600 plant species. In this study, among the 106,893 transcripts in the head assembly, 8 OBPs and 13 CSPs were identified in B. tabaci MED based on head transcriptomes of adults. Phylogenetic analyses were conducted to investigate the relationships of B. tabaci OBPs and CSPs with those from several other important Hemipteran species, and the motif-patterns between Hemiptera OBPs and CSPs were also compared by MEME. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MED adults were analyzed by real-time qPCR. Seven out of the 8 OBPs found in B. tabaci MED were highly expressed in the head. Conversely, only 4 CSPs were enriched in the head, while the other nine CSPs were specifically expressed in other tissues. Our findings pave the way for future research on chemical recognition of B. tabaci at the molecular level.
节肢动物的气味结合蛋白（OBPs）和化学感受蛋白（CSPs）被认为参与化学识别，这种识别调节着包括宿主选择、交配和繁殖在内的关键行为。在昆虫中，OBPs和CSPs主要位于触角中，但在烟粉虱中尚未进行系统的表征，烟粉虱是一种隐存种复合体，可危害600多种植物。在本研究中，基于成虫的头部转录组，在烟粉虱MED的头部组装体的106,893个转录本中，鉴定出8种OBPs和13种CSPs。进行系统发育分析以研究烟粉虱OBPs和CSPs与其他几种重要半翅目物种的关系，并通过MEME比较半翅目OBPs和CSPs之间的基序模式。通过实时定量PCR分析了烟粉虱MED成虫不同组织中OBP和CSP基因的表达谱。在烟粉虱MED中发现的8种OBPs中有7种在头部高表达。相反，只有4种CSPs在头部富集，而其他9种CSPs在其他组织中特异性表达。我们的研究结果为今后在分子水平上研究烟粉虱的化学识别奠定了基础。
