Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO), Carretera de la Azohía s/n, Puerto de Mazarrón, 30860, Murcia, Spain.
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Sci Rep. 2017 Nov 13;7(1):15396. doi: 10.1038/s41598-017-15629-6.
Nervous necrosis virus (NNV) causes high mortalities in several marine species. We aimed to evaluate the innate cell-mediated cytotoxic (CMC) activity of head-kidney leucocytes (HKLs) isolated from naïve European sea bass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), a very susceptible and resistant fish species to NNV, respectively, against fish cell lines infected with NNV. Seabream HKLs showed significantly increased innate CMC activity against NNV-infected cells, compared to those uninfected, while sea bass HKLs failed to do so. Thus, we performed a RNA-seq study to identify genes related to the CMC activity of sea bass leucocytes. Thus, we found that sea bass HKLs incubated with DLB-1 cells alone (CMC_DLB1) or with NNV-infected DLB-1 cells (CMC_DLB1-NNV) showed very similar transcriptomic profiles and the GO analysis revealed that most of the up-regulated genes were related to immunity. Strikingly, when the CMC samples with and without NNV were compared, GO analysis revealed that most of the up-regulated genes in CMC_DLB1-NNV samples were related to metabolism and very few to immunity. This is also in agreement with the functional data. These data point to the escape of CMC activity by NNV infection as an important factor involved in the high susceptibility to nodavirus infections of European sea bass.
神经坏死病毒（NNV）可导致多种海洋物种死亡率升高。我们旨在评估分别对 NNV 高度易感和抗性的鱼类欧洲鲈鱼（Dicentrarchus labrax）和金头鲷（Sparus aurata）的头肾白细胞（HKL）固有细胞介导的细胞毒性（CMC）活性，以针对 NNV 感染的鱼细胞系。与未感染的细胞相比，金头鲷 HKL 对 NNV 感染细胞的固有 CMC 活性明显增加，而鲈鱼 HKL 则没有。因此，我们进行了 RNA-seq 研究，以鉴定与鲈鱼白细胞 CMC 活性相关的基因。因此，我们发现单独孵育 DLB-1 细胞（CMC_DLB1）或 NNV 感染的 DLB-1 细胞（CMC_DLB1-NNV）的鲈鱼 HKL 显示出非常相似的转录组图谱，GO 分析表明，上调的基因大多与免疫有关。引人注目的是，当比较有和没有 NNV 的 CMC 样本时，GO 分析表明，CMC_DLB1-NNV 样本中上调的基因大多数与代谢有关，而与免疫有关的基因很少。这也与功能数据一致。这些数据表明，NNV 感染导致的 CMC 活性逃逸是欧洲鲈鱼对诺达病毒感染高度易感的一个重要因素。
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